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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Localization of a bacterial protein in starch granules of transgenic maize kernels
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Localization of a bacterial protein in starch granules of transgenic maize kernels

机译：转基因玉米粒淀粉颗粒中细菌蛋白的定位

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The B subunit of Escherichia coli heat labile enterotoxin (LT-B) is a potent oral immunogen with potential for use as a vaccine, a carrier molecule to deliver antigens to gut-associated lymphoid tissues, and possibly an adjuvant to make coadministered vaccines more effective. LT-B produced in plants was shown to be functional and immunogenic in animals and humans. In this work, we show that maize-derived LT-B is strongly associated with starch granules in endosperm. Using immunogold labeling/electron microscopy, cell fractionation, and protein analysis techniques, we observed that LT-B protein could be detected both internally and externally in starch granules. This strong association confers an effective copurification of the antigen with the starch fraction of maize kernels, thermostability desirable in maize processing, and resistance to peptic degradation in simulated gastric fluid digests, an important attribute for an orally delivered antigen. [References: 32]
机译：大肠杆菌热不稳定肠毒素（LT-B）的B亚基是一种有效的口服免疫原，具有用作疫苗的潜能，将抗原递送至肠道相关淋巴组织的载体分子以及可能使佐剂更有效的佐剂。已证明在植物中产生的LT-B在动物和人类中具有功能和免疫原性。在这项工作中，我们证明了玉米衍生的LT-B与胚乳中的淀粉颗粒紧密相关。使用免疫金标记/电子显微镜，细胞分级分离和蛋白质分析技术，我们观察到可以在淀粉颗粒的内部和外部检测到LT-B蛋白。这种强的结合赋予抗原与玉米粒的淀粉级分的有效共纯化，玉米加工所需的热稳定性以及对模拟胃液消化液中消化性降解的抵抗力，这是口服递送抗原的重要属性。 [参考：32]

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