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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dissection of K~+ currents in Caenorhabditis elegans muscle cells by genetics and RNA interference
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Dissection of K~+ currents in Caenorhabditis elegans muscle cells by genetics and RNA interference

机译：秀丽隐杆线虫肌细胞中K〜+电流的遗传学和RNA干扰分析

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摘要

GFP-promoter experiments have previously shown that at least nine genes encoding potassium channel subunits are expressed in Caeno-rhabditis elegans muscle. By using genetic, RNA interference, and physiological techniques we revealed the molecular identity of the major components of the outward K~+ currents in body wall muscle cells in culture. We found that under physiological conditions, outward current is dominated by the products of only two genes. Shaker (Kv1) and Shal (Kv4), both expressing voltage-dependent potassium channels. Other channels may be held in reserve to respond to particular circumstances. Because GFP-promoter experiments indicated that slo-2 expression is prominent, we created a deletion mutant to identify the SLO-2 current in vivo. In both whole-cell and single-channel modes, in vivo SLO-2 channels were active only when intracellular Ca~(2+) and Cl~- were raised above normal physiological conditions, as occurs during hypoxia. Under such conditions, SLO-2 is the largest outward current, contributing up to 87% of the total current. Other channels are present in muscle, but our results suggest that they are unlikely to contribute a large outward component under physiological conditions. However, they, too, may contribute currents conditional on other factors. Hence, the picture that emerges is of a complex membrane with a small number of household conductances functioning under normal circumstances, but with additional conductances that are activated during unusual circumstances.
机译：GFP启动子实验以前已经表明，在秀丽隐杆线虫肌肉中至少有9个编码钾通道亚基的基因表达。通过使用遗传，RNA干扰和生理学技术，我们揭示了培养物中体壁肌肉细胞中外向钾离子电流主要成分的分子同一性。我们发现在生理条件下，外向电流仅由两个基因的产物决定。摇床（Kv1）和沙尔（Kv4）均表达电压依赖性钾离子通道。可以保留其他渠道以应对特定情况。因为GFP启动子实验表明slo-2的表达是突出的，所以我们创建了一个缺失突变体来识别SLO-2当前在体内。在全细胞和单通道模式下，体内SLO-2通道仅在细胞内Ca〜（2+）和Cl〜-升高至正常生理条件以上时才激活，如在缺氧期间发生的情况。在这种情况下，SLO-2是最大的向外电流，占总电流的87％。肌肉中还存在其他通道，但我们的结果表明，在生理条件下，它们不太可能贡献大量的向外成分。但是，它们也可能以其他因素为条件贡献电流。因此，出现的图像是复杂的膜，在正常情况下具有少量的家庭电导，而在异常情况下会激活额外的电导。

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