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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures
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Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures

机译：微染色体维持蛋白4、6和7复合物的进行性DNA解旋酶活性需要分叉的DNA结构

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摘要

The minichromosome maintenance (Mcm) proteins Z--7 are re- quired for both the initiation and elongation steps of chromosomal DNA replication. Previous studies have shown that the Mcm complex consisting of the Mcm 4, 6. and 7 proteins contains 3' to 5' DNA helicase activity with limited processivity (displacing duplex DNA regions up to 30 nt). In this report, we show that the presence of both 5' and 3' single-stranded tails in DNA helicase substrates is essential for the processive helicase activity of the Mcm complex. The presence of both 5' and 3' tails facilitated the formation of double heterohexameric complexes of Mcm4/6/7 on substrate DNA, which appeared to be essential for the processive helicase activity. The double heterohexameric complex of Mcm4/6/7. in the presence of a single-strand DNA binding protein, is capable of unwinding duplex DNA region of about 600 bp in length. These results support the hypothesis that the Mcm4/6/7 complex can function as a replication helicase.
机译：染色体DNA复制的起始和延伸步骤都需要微染色体维持蛋白（Mcm）Z--7。先前的研究表明，由Mcm 4、6和7蛋白组成的Mcm复合物包含3'至5'DNA解旋酶活性，且具有有限的合成能力（取代双链DNA区域可达30 nt）。在此报告中，我们表明DNA解旋酶底物中同时存在5'和3'单链尾部对于Mcm复合体的进行性解旋酶活性至关重要。 5'和3'尾部的存在促进了底物DNA上Mcm4 / 6/7的双重异六聚体复合物的形成，这似乎对进行性解旋酶活性至关重要。 Mcm4 / 6/7的双异六聚体复合物。在存在单链DNA结合蛋白的情况下，其能够解开长度约为600bp的双链DNA区域。这些结果支持Mcm4 / 6/7复合体可以充当复制解旋酶的假设。

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