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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Blocking histone deacetylation in ArabidopsiS induces pleiotropic effects on plant gene regulation and development
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Blocking histone deacetylation in ArabidopsiS induces pleiotropic effects on plant gene regulation and development

机译：阻止拟南芥中的组蛋白去乙酰化诱导多效性对植物基因调控和发育的影响

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Histone acetylation and deacetylation play essential roles in eu- karyotic gene regulation. Reversible modifications of core histones are catalyzed by two intrinsic enzymes. histone acetyltransferase and histone deacetylase (HD). In general, histone deacetylation is related to transcriptional gene silencing, whereas acetylation cor- relates with gene activation. We produced transgenic plants ex- pressing the antisense Arabbopsis HD (AtHD1) gene. AtHD1 is a homolog of human HD1 and RPD3 global transcriptional regulator in yeast. Expression of the antisense AtHD1 caused dramatic reduction in endogenous AtHD1 transcription, resulting in accu- mulation of acetylated histones, notably tetraacetylated H4. Re- duction in AtHD1 expression and AtHD1 production and changes in acetylation profiles were associated with various developmental abnormalities. including early senescence, ectopic expression of silenced genes, suppression of apical dominance, homeotic changes. heterochronic shift toward juvenility, flower defects, and male and female sterility. Some of the phenotypes could be attributed to ectopic expression of tissue-specific genes (e.g., SUPRMAN) in vegetative tissues. No changes in genomic DNA methylation were detected in the transgenic plants. These results suggest that AtHD1 is a global regulator, which controls gene expression during development through DNA-sequence indepen- dent or epigenetic mechanisms in plants. In addition to DNA methylation, histone modifications may be involved in a general regulatory mechanism responsible for plant plasticity and variation in nature.
机译：组蛋白乙酰化和脱乙酰化在真核基因调控中起着至关重要的作用。核心组蛋白的可逆修饰被两种内在酶催化。组蛋白乙酰转移酶和组蛋白脱乙酰酶（HD）。通常，组蛋白去乙酰化与转录基因沉默有关，而乙酰化与基因激活有关。我们生产了表达反义拟南芥HD（AtHD1）基因的转基因植物。 AtHD1是酵母中人HD1和RPD3全球转录调节因子的同源物。反义AtHD1的表达引起内源性AtHD1转录的急剧减少，从而导致乙酰化组蛋白（尤其是四乙酰化H4）的积累。 AtHD1表达和AtHD1产生的减少以及乙酰化曲线的改变与各种发育异常有关。包括早期衰老，沉默基因的异位表达，根尖抑制，顺势变化。朝着青春期，花朵缺陷以及雄性和雌性不育的异时性转变。一些表型可归因于在营养组织中组织特异性基因（例如SUPRMAN）的异位表达。在转基因植物中未检测到基因组DNA甲基化的变化。这些结果表明AtHD1是一种全局调节剂，通过植物中的DNA序列独立或表观遗传机制来控制发育过程中的基因表达。除DNA甲基化外，组蛋白修饰可能参与植物可塑性和自然变异的一般调控机制。

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