• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Characterization of Rny1, the Saccharomyces cerevisiae member of the T2 RNase family of RNases: Unexpected functions for ancient enzymes?
【24h】

Characterization of Rny1, the Saccharomyces cerevisiae member of the T2 RNase family of RNases: Unexpected functions for ancient enzymes?

机译：Rny1的特性，RNases T2 RNase家族的酿酒酵母成员：古代酶的意外功能？

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The T2 family of nonspecific endoribonucleases (EC 3.1.27.1) is a widespread family of RNases found in every organism examined thus far. Most T_2 enzymes are secretory RNases and therefore are found extracellularly or in compartments of the endomembrane system that would minimize their contact with cellular RNA. Although the biological functions of various T_2 RNases have been postulated on the basis of enzyme location or gene expression patterns, the cellular roles of these enzymes are generally un- known. In the present work, we characterized Rny1, the only T_2 RNase in Saccharomyces cerevisiae. Rny1 was found to be an active, secreted RNase whose gene expression is controlled by heat shock and osmotic stress. Inactivation of RNY1 leads to unusually large cells that are temperature-sensitive for growth. These phenotypes can be complemented not only by RNY1 but also by both struc- turally related and unrelated secretory RNases. Additionally, the complementation depends on RNase activity. When coupled with a recent report on the effect of specific RNAs on membrane permeability [Khvorova, A., Kwak, Y-G., Tamkun, M., Majerfeld, l. & Yarus, M. (1999) Proc. Natl. Acad. Sci. USA 96, 10649-10654], our work suggests an unexpected role for Rny1 and possibly other secretory RNases. These enzymes may regulate membrane perme- ability or stability, a hypothesis that could present an alternative perspective for understanding their functions.
机译：T2家族的非特异性内切核糖核酸酶（EC 3.1.27.1）是迄今为止在每个受检生物中发现的广泛的RNase家族。大多数T_2酶是分泌型RNase，因此被发现在细胞外或内膜系统的区室中，这将使它们与细胞RNA的接触减至最少。尽管已经根据酶的位置或基因表达模式推测了各种T_2 RNase的生物学功能，但是这些酶的细胞作用通常是未知的。在目前的工作中，我们表征了酿酒酵母中唯一的T_2 RNase Rny1。发现Rny1是一种活跃的分泌型RNase，其基因表达受热激和渗透压控制。 RNY1的失活导致异常大的细胞对生长敏感。这些表型不仅可以通过RNY1进行补充，而且可以通过结构相关和不相关的分泌性RNase进行补充。另外，互补取决于RNase活性。当结合有关特定RNA对膜通透性影响的最新报道时[Khvorova，A.，Kwak，Y-G。，Tamkun，M.，Majerfeld，1。 ＆Yarus，M.（1999）Proc。 Natl。学院科学[USA 96，10649-10654]，我们的工作表明Rny1可能还有其他分泌型RNase的意外作用。这些酶可能调节膜的通透性或稳定性，这一假说可能为理解其功能提供了另一种观点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号