...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopus oocytes
【24h】

Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopus oocytes

机译：表面定位的甘氨酸转运蛋白1和2在非洲爪蟾卵母细胞中起单体蛋白的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Na~+/Cl~- -dependent neurotransmitter transporters form a super- family of transmembrane proteins that share 12 membrane-span- ning regions. To gain information about the quaternary structure of these transporter proteins, we heterologously expressed the glial glycine transporter GlyT1 and its neuronal homolog GlyT2 in Xenopus oocytes. By using metabolic labeling with [~35S]methi- onine or surface labeling with a plasma membrane impermeable reagent followed by affinity purification, we separately analyzed the total cellular pools of newly synthesized GlyTs and its func- tional plasma membrane-bound fractions. Upon blue native gel electrophoresis, the surface-localized transporter proteins were found to exist exclusively in complex-glycosylated monomeric form, whereas a significant fraction of the intracellular GlyT1 and GlyT2 was core-glycosylated and oligomeric. In contrast, even after treatment with the crosslinker glutaraldehyde, surface GlyTs failed to migrate as oligomeric proteins. These results indicate that plasma membrane-bound GlyT1 and GlyT2 are monomeric pro- teins. Thus, Na~+/Cl~- -dependent neurotransmitter transporters do not require oligomerization for substrate translocation.
机译：Na〜+ / Cl〜-依赖的神经递质转运蛋白形成跨膜蛋白超家族，共有12个跨膜区域。为了获得有关这些转运蛋白的四级结构的信息，我们在非洲爪蟾卵母细胞中异源表达了神经胶质甘氨酸转运蛋白GlyT1及其神经元同源物GlyT2。通过使用[〜35S]甲硫氨酸进行代谢标记或使用质膜不渗透试剂进行表面标记，然后进行亲和纯化，我们分别分析了新合成的GlyT及其功能膜结合部分的总细胞池。在蓝色天然凝胶电泳中，发现表面定位的转运蛋白仅以复合糖基化单体形式存在，而细胞内GlyT1和GlyT2的很大一部分是核心糖基化和寡聚体。相反，即使在用交联剂戊二醛处理后，表面GlyTs也不能作为寡聚蛋白迁移。这些结果表明，质膜结合的GlyT1和GlyT2是单体蛋白。因此，依赖Na + + / Cl--的神经递质转运蛋白不需要寡聚来进行底物转运。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号