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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Expression of the Anabaena hetR gene from a copper-regulated promoter leads to heterocyst differentiation under repressing conditions
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Expression of the Anabaena hetR gene from a copper-regulated promoter leads to heterocyst differentiation under repressing conditions

机译：来自铜调控启动子的鱼腥藻hetR基因的表达导致在抑制条件下异胚囊分化

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摘要

Heterocyst differentiation in the filamentous cyanobacterium Anabaena PCC 7120 requires a functional hetR gene. Increased expression of the hetR gene is seen in developing and mature heterocysts in response to fixed nitrogen limitation. We mapped four likely transcriptional start sites for hetR and identified a specific transcript that is positively autoregulated. By using the capper-responsive petE promoter from Anabaena PCC 7120 to drive hetR expression, we show that ectopic expression of hetR increases heterocyst frequency and induces heterocyst differenti- ation under fully repressing conditions. Coexpression of a reporter gene shows that expression from the petE promoter is smoothly induced depending on the amount of copper supplied. In the heterocyst pattern mutant PatA, where terminally positioned het- erocysts are formed almost exclusively, expression of the petE::hetR fusion does not result in the formation of intercalary heterocysts. These results suggest that although the intracellular concentration of HetR has to be elevated for the differentiation decision. PatA plays a role as well. This role may be in the form of posttranslational modification of HetR, because PatA is a member of the response regulator family of proteins.
机译：丝状蓝细菌鱼腥藻PCC 7120中的异质囊分化需要功能性hetR基因。响应固定的氮限制，在发育中和成熟的异型囊中见到了hetR基因表达的增加。我们为hetR绘制了四个可能的转录起始位点，并确定了一个可以自动调节的特定转录物。通过使用来自Anabaena PCC 7120的capper响应性petE启动子来驱动hetR表达，我们显示异位表达的hetR在完全抑制的条件下增加了异胞体的频率并诱导了异胞体的分化。报告基因的共表达表明，根据所供应的铜的量，来自petE启动子的表达被平滑地诱导。在异种模式的突变体PatA中，几乎仅形成末端定位的异型囊肿，petE :: hetR融合蛋白的表达不会导致cal间异型囊的形成。这些结果表明，尽管必须提高HetR的细胞内浓度才能做出分化决定。 PatA也起着作用。该作用可能是HetR的翻译后修饰形式，因为PatA是蛋白质反应调节因子家族的成员。

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