Cryoelectron microscopy of λ phage DNA condensates in vitreous ice: The fine structure of DNA toroids

Nicholas V. Hud; Kenneth H. Downing

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Cryoelectron microscopy of λ phage DNA condensates in vitreous ice: The fine structure of DNA toroids

机译：玻璃冰中λ噬菌体DNA冷凝物的低温电子显微镜：DNA环的精细结构

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DNA toroids produced by the condensation of λ phage DNA with hexammine cobalt (III) have been investigated by cryoelectron microscopy. Image resolution obtained by this technique has al- lowed unprecedented views of DNA packing within toroidal con- densates. Toroids oriented coplanar with the microscope image plane exhibit circular fringes with a repeat spacing of 2.4 nm. For some toroids these fringes are observed around almost the entire circumference of the toroid. However, for most toroids well- defined fringes are limited to less than one-third of the total toroid circumference.

机译：已经通过冷冻电子显微镜研究了由λ噬菌体DNA与六乙胺钴（III）缩合产生的DNA环。通过这种技术获得的图像分辨率使人们对环形浓缩物中DNA的堆积有了空前的看法。与显微镜图像平面共面的环形线圈显示出圆形条纹，重复间距为2.4 nm。对于某些环形线圈，这些条纹几乎可以在环形线圈的整个圆周上观察到。但是，对于大多数环面，轮廓分明的条纹被限制为小于环面总圆周的三分之一。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第26期|p.14925-14930|共6页
作者
Nicholas V. Hud; Kenneth H. Downing;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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2. Structure of toroidal DNA collapsed inside the phage capsid [J] . Amelie Leforestier, Francoise Livolant Proceedings of the National Academy of Sciences of the United States of America . 2009,第23期

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3. Fine mapping of secondary structures of fd phage DNA in the region of the replication origin [J] . Chiao-Chain Huang, John E. Hearst Nucleic acids research . 1981,第21期

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5. Cryoelectron microscopy studies of low-density lipoprotein in vitreous ice. [D] . Spin, Joshua Michael. 1997

机译：玻璃冰中低密度脂蛋白的低温电子显微镜研究。
6. From the Cover: Cryoelectron microscopy of λ phage DNA condensates in vitreous ice: The fine structure of DNA toroids [O] . Nicholas V. Hud, Kenneth H. Downing 2001

机译：从封面：玻璃体中λ噬菌体DNA冷凝物的低温电子显微镜冰：DNA环的精细结构
7. Cryoelectron microscopy of λ phage DNA condensates in vitreous ice: The fine structure of DNA toroids [O] . Hud, Nicholas V., Downing, Kenneth H. 2001

机译：玻璃体中λ噬菌体DNA冷凝物的低温电子显微镜冰：DNA环的精细结构
8. Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing. Final report, June 1, 1988--January 31, 1996 [R] . Richardson, C. C. 1996

机译：表征和修饰用于DNa测序的噬菌体T7 DNa聚合酶。最终报告，1988年6月1日至1996年1月31日

Cryoelectron microscopy of λ phage DNA condensates in vitreous ice: The fine structure of DNA toroids

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