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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Continuous in vitro propagation and differentiation of cultares of the intramolluscan stages of the human parasite Schistosoma mansonl
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Continuous in vitro propagation and differentiation of cultares of the intramolluscan stages of the human parasite Schistosoma mansonl

机译：人类寄生虫血吸虫的软体动物内阶段的培养物的连续体外繁殖和分化

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摘要

The metazoan parasitic blood flukes, Schis- tosoma spp-, infect over 200 million people worldwide and cause extensive human morbidity and mortality. Research strategies for development of anti-schistosomal agents are impeded by the organism's complex molluscan-mammalian life cycle, which limits experimental approaches and avail- ability of material. We derived long-term continuously pro- liferative cultures of Schistosoma mansoni sporocysts capable of generating cercariae in vitro. Cultured organisms retained the ability to parasitize the host, and they exhibited develop- mental regulation of candidate stage-specific genes in the host-free culture system. Evidence for expression of a reverse transcriptase also was found in the cultured organisms, pointing to this activity as a possible mechanistic contributor to the dynamic relationship between the parasite and its hosts. Continuous in vitro propagation of the asexual sporocyst stage allows isolation of clonally derived parasite populations and provides a means to study schistosomal molecular genetics, metabolism, and evasion of host defenses.
机译：后生寄生虫血吸虫（Schistosoma spp-）感染了全世界2亿多人，并导致广泛的人类发病率和死亡率。生物体复杂的软体动物-哺乳动物生命周期阻碍了抗血吸虫病药物的研发策略，这限制了实验方法和材料的利用率。我们获得了能够在体外产生尾c的曼氏血吸虫孢子囊的长期连续增生培养物。培养的生物保留了寄生宿主的能力，并且在无宿主培养系统中表现出候选阶段特异性基因的发育调控。在培养的生物体中也发现了表达逆转录酶的证据，指出这种活性可能是寄生虫与其宿主之间动态关系的可能机制。无性孢子囊期的连续体外繁殖可以分离出克隆来源的寄生虫种群，并提供了一种研究血吸虫分子遗传学，代谢和逃避宿主防御的手段。

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