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首页> 外文期刊>Science in China. Series C, Life sciences >Preliminary study on the molecular structure of 3' region of Duchenne muscular dystrophy (DMD) gene in Chinese
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Preliminary study on the molecular structure of 3' region of Duchenne muscular dystrophy (DMD) gene in Chinese

机译：中国人杜氏肌营养不良（DMD）基因3'区分子结构的初步研究

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摘要

Number and order of HindIII exon-containing fragments (Hd) at 3' region of DMD gene were studied systematically using 16 partly-overlapping cDNA subprobes which were produced from dystrophin cDNA 9—14 with each of 9 restriction endonucleases. There are 25 Hd fragments corresponding to cDNA 9—14 in DMD gene. Since then, the exact length and the new order of Hd fragments are established. A new 2.1 kb fragment (Hd 55) is revealed; a 5.2 kb fragment (formely designated as Hd 59) is excluded and the existence of a controversial 3.2 kb fragment (Hd 64) is confirmed. Besides, three new exons were revealed by comparing the PvuII and the Xbal hybridization patterns with the Hindlll hybridization patterns for these cDNA subprobes. It is concluded that thereare at least 66 Hd fragments, or 79 exons in DMD gene basing on the discovery of three additional exons. The corresponding relationship between the 66 Hd fragments and the SfiI large scale physical map has been studied, and at least 17 Hd fragments or 19 exons were shown to be distributed in the last fragment (II fragment) of the Sfil map of DMD gene.
机译：使用抗肌钙蛋白cDNA 9-14产生的16个部分重叠的cDNA亚探针，用9种限制性核酸内切酶中的每一种，系统地研究了DMD基因3'区域含HindIII外显子的片段（Hd）的数量和顺序。 DMD基因中有25个Hd片段对应于cDNA 9-14。从那时起，确定了Hd片段的确切长度和​​新顺序。揭示了一个新的2.1 kb片段（Hd 55）；排除了一个5.2 kb片段（以前称为Hd 59），并确认存在争议的3.2 kb片段（Hd 64）。此外，通过比较PvuII和Xbal杂交模式与这些cDNA亚探针的HindIII杂交模式，揭示了三个新的外显子。结论是，基于另外三个外显子的发现，DMD基因中至少有66个Hd片段或79个外显子。研究了66个Hd片段与SfiI大规模物理图谱之间的对应关系，并显示至少有17个Hd片段或19个外显子分布在DMD基因Sfil图谱的最后一个片段（II片段）中。

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