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Optically Based Hand‑Held Sensor for Visualization and Quantification of Cryptosporidium Parvum

机译：用于可视化和定量隐孢子虫的镜持的传感器

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摘要

The ingestion of the protozoan parasite Cryptosporidium, resulting in the associated disease cryptosporidiosis, can cause major problems if introduced into a water supply for both animals and humans. This research focuses on the creation of a sensor to track and quantify the parasite with a digital camera system using Digital Inline Holographic Microscopy (DIHM) and software reconstruction. Since Cryptosporidium oocysts are not single celled and can be around 4–8 μm in diameter, they are easily detected with the DIHM at various depths within a 3.5 mL cuvette. As the sensor is improved by modifications, it could pick up behavior of movement and sizes of particles measured to positively identify the particle(s) being tracked as Cryptosporidium as others have achieved on Escherichia coli and other microorganisms. A significant advantage of this technique is the resulting sensor costs significantly less than traditional microscopes used in laboratory tests for detecting Cryptosporidium, or other methods like USEPA Method 1623 and can provide results within minutes. The sensor is a stellar alternative to current methods in detecting and quantifying Cryptosporidium.
机译：摄入原生动物寄生虫密码孢子率，导致相关的疾病密码刺激症，如果引入动物和人类的供水中会产生重大问题。该研究侧重于使用数字内联全息显微镜（DIHM）和软件重建的数码相机系统创建传感器以跟踪和量化寄生虫。由于密码孢子鎓卵囊不是单一的细胞，并且直径可以是约4-8μm的，因此在3.5ml比色皿内的各种深度处容易检测它们。随着传感器的修饰得到改善，它可以拾取测量的颗粒的运动和尺寸的行为，以肯定地识别被追踪作为密码孢子率的颗粒，因为其他在大肠杆菌和其他微生物上已经实现的颗粒。该技术的显着优点是得到的传感器成本明显小于用于检测隐孢子虫的实验室测试中使用的传统显微镜，或者如US​​PA方法1623等其他方法，并且可以在分钟内提供结果。传感器是检测和量化密码孢子率的当前方法的恒星替代方案。

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  • 来源
    《Sensing and imaging》 |2020年第1期|46.1-46.14|共14页
  • 作者

    Andrew Thompson; Basil Hable; Kyana Young; Thomas Hansen; J. R. Strickler; Marcia R. Silva;

  • 作者单位

    Water Technology Accelerator (WaTA) University of Wisconsin-Milwaukee Milwaukee WI USA;

    Water Technology Accelerator (WaTA) University of Wisconsin-Milwaukee Milwaukee WI USA;

    Department of Engineering Wake Forest University Winston‑Salem NC USA;

    Water Technology Accelerator (WaTA) University of Wisconsin-Milwaukee Milwaukee WI USA School of Freshwater Sciences University of Wisconsin-Milwaukee Milwaukee WI USA;

    Water Technology Accelerator (WaTA) University of Wisconsin-Milwaukee Milwaukee WI USA Department of Biological Sciences University of Wisconsin-Milwaukee Milwaukee WI USA;

    Water Technology Accelerator (WaTA) University of Wisconsin-Milwaukee Milwaukee WI USA;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Cryptosporidium; Sensor; DIHM; Water;

    机译：隐孢子虫;传感器;DIHM;水;

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