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首页> 外文期刊>Sensors and Actuators >Imaging of the mutual regulation between zinc cation and nitrosyl via two-photon fluorescent probes in cells and in vivo
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Imaging of the mutual regulation between zinc cation and nitrosyl via two-photon fluorescent probes in cells and in vivo

机译：通过双光子荧光探针和体内通过双光子荧光探针对阳离子和亚硝基相互调节的成像

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摘要

The homeostatic disorder of intracellular Zn~(2+) pool is closely associated with severe diseases. It has been reported that the high level of free Zn~(2+) during ischemia/reperfusion (I/R) process can result in oxidative stress damage on nerve cells. Given that nitrosyl (HNO) can aggravate the nerve injury during cerebral I/R process, we assume that there may exist a mutual regulation between Zn~(2+) and HNO under certain physiological conditions. To reveal this potential small-signaling-molecule crosstalk, we synthesized two-photon fluorescent probes CHP-H and CHP-CH_3 to monitor intracellular Zn~(2+) in cell and mice hippocampus I/R models. The probes consist of two moieties: coumarin derivative as the two-photon fluorescence transducer, 2-hydrazino pyridine as the fluorescence modulator and Zn~(2+) chelator. Both probes exhibit excellent analytical properties for Zn~(2+) detection in simulated physiological systems. Utilizing CHP-H and an HNO probe Cyto-JN, we perform fluorescent imaging of cell I/R models. The results confirm that HNO can stimulate Zn~(2+) release from labile Zn~(2+) pool, whereas, the increase of intracellular Zn~(2+) cannot upregulate the level of HNO. Combining with the deep tissue imaging of mice hippocampus tissues, our probes may provide potential approaches for the medical diagnostic assessment of HNO regulation effect on Zn~(2+) release in clinical cerebral I/R-related diseases.
机译：细胞内Zn〜（2+）池的稳态障碍与严重疾病密切相关。据报道，在缺血/再灌注（I / R）过程中的高水平的游离Zn〜（2+）可导致神经细胞造成氧化应激损伤。鉴于亚硝基（HNO）可以在脑I / R过程中加重神经损伤，假设在某些生理条件下可能存在Zn〜（2+）和HNO之间的相互调节。为了揭示这种潜在的小信令分子串扰，我们合成了双光子荧光探针CHP-H和CHP-CH_3以监测细胞和小鼠海马I / R模型中的细胞内Zn〜（2+）。探针由两部分组成：香豆素衍生物作为双光子荧光传感器，2-肼吡啶作为荧光调节剂和Zn〜（2+）螯合剂。两种探针在模拟生理系统中表现出优异的分析性质用于Zn〜（2+）检测。利用CHP-H和HNO探针CYTO-JN，我们执行细胞I / R模型的荧光成像。结果证实，HNO可以从不稳定的Zn〜（2+）池中刺激Zn〜（2+）释放，而细胞内Zn〜（2+）的增加不能上调HNO的水平。与小鼠海马组织的深层组织成像组合，我们的探针可以为临床脑I / R相关疾病中对Zn〜（2+）释放的HNO调控作用的医学诊断评估的潜在方法。

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  • 来源
    《Sensors and Actuators》 |2020年第4期|127772.1-127772.9|共9页
  • 作者

    Mingshun Li; Yanlong Xing; Yuxia Zou; Guang Chen; Jinmao You; Fabiao Yu;

  • 作者单位

    The Key Laboratory of Life-Organic Analysis Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine College of Chemistry and Chemical Engineering Qufu Normal University Qufu 273165 China;

    Institute of Functional Materials and Molecular Imaging Key Laboratory of Emergency and Trauma Ministry of Education Key Laboratory of Hainan Trauma and Disaster Rescue College of Clinical Medicine College of Emergency and Trauma Hainan Medical University Haikou 571199 China;

    Institute of Functional Materials and Molecular Imaging Key Laboratory of Emergency and Trauma Ministry of Education Key Laboratory of Hainan Trauma and Disaster Rescue College of Clinical Medicine College of Emergency and Trauma Hainan Medical University Haikou 571199 China;

    The Key Laboratory of Life-Organic Analysis Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine College of Chemistry and Chemical Engineering Qufu Normal University Qufu 273165 China;

    The Key Laboratory of Life-Organic Analysis Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine College of Chemistry and Chemical Engineering Qufu Normal University Qufu 273165 China;

    The Key Laboratory of Life-Organic Analysis Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine College of Chemistry and Chemical Engineering Qufu Normal University Qufu 273165 China Institute of Functional Materials and Molecular Imaging Key Laboratory of Emergency and Trauma Ministry of Education Key Laboratory of Hainan Trauma and Disaster Rescue College of Clinical Medicine College of Emergency and Trauma Hainan Medical University Haikou 571199 China;

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  • 正文语种 eng
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  • 关键词

    Fluorescent probes; Two-photon; Zinc cation; Nitrosyl; Cell imaging; In vivo imaging;

    机译：荧光探针;双光子;锌阳离子;亚硝基;细胞成像;在体内成像;

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