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首页> 外文期刊>Sensors and Actuators >An 'off-on' fluorescein-based colormetric and fluorescent probe for the detection of glutathione and cysteine over homocysteine and its application for cell imaging
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An 'off-on' fluorescein-based colormetric and fluorescent probe for the detection of glutathione and cysteine over homocysteine and its application for cell imaging

机译：一种基于荧光素的“开-关”比色法和荧光探针，用于检测同型半胱氨酸上的谷胱甘肽和半胱氨酸，并将其用于细胞成像

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摘要

A new fluorescein-based probe (Py-F) was designed and synthesized to develop an optical and fluorescent sensor for biological thiols.Py-Fpossessed the thiol-responsed disulfide group and displayed selectivity and sensitivity for glutathione (GSH) and cysteine (Cys) over homocysteine (Hcy) in the CH3CN/PBS buffer solution, and the fluorescence detection limit was calculated to be 0.12 μM and 0.13 μM, respectively. Besides, the recognition mechanism was explained by density functional theory (DFT) calculation, and confirmed by mass spectroscope measurement. Furthermore, the thiols detection application in cellular imaging assays indicated that the probe has appreciable cell permeability and can serve as a fluorescent probe for detecting biothiols in living cells.
机译：设计并合成了一种新的基于荧光素的探针（Py-F），以开发一种用于生物硫醇的光学和荧光传感器。 CH3CN / PBS缓冲液中的高半胱氨酸（Hcy）的荧光检测限分别为0.12μm和0.13μm。此外，通过密度泛函理论（DFT）计算解释了识别机理，并通过质谱仪测量证实了这一机理。此外，硫醇检测在细胞成像分析中的应用表明该探针具有明显的细胞通透性，可以用作检测活细胞中生物硫醇的荧光探针。

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  • 来源
    《Sensors and Actuators》 |2018年第5期|295-302|共8页
  • 作者

    Xufeng Hou; Zhensheng Li; Bolun Li; Chunhui Liu; Zhihong Xu;

  • 作者单位

    Key laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University;

    Key laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University;

    Key laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University;

    Key laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University;

    Key laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Fluorescein; Fluorescent probe; Glutathione; Cysteine; Cell imaging;

    机译：荧光素;荧光探针;谷胱甘肽;半胱氨酸;细胞成像;

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