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Rapid Method Development of HPLC by Using Ultra Fast LC System

机译：使用超快速液相色谱系统快速开发HPLC方法

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It is worth to note that linear method transfer between fast LC (UFLC) and conventional HPLC is critical in this application. A good linear relationship observed in this work may be related to two factors, the same types of C18 columns (manufacturer, materials, packing and capping technique, etc) and on the same system for both UFLC column and HPLC column. These factors minimize the differences of chromatographic conditions except the particle size of packing materials and dimension of the columns. We have not tried the same procedure on different types of columns and different systems. It is expected, however, that the differences in LC systems may cause systematic shift of retention times, but would not affect the linear relationship between fast LC and conventional HPLC if the system configurations are same and the piping systems are well set up to minimize dead volumes. It has not examined the linear method transfer relationship among columns provided by different manufacturers. Further study on this aspect is needed.rnThe results of this work show that UFLC could be very useful in HPLC method development to enhance the efficiency and productivity. This approach is practically beneficial to various industries like chemicals, foods and pharmaceutics where conventional HPLC is still dominant in their QA/QC and R&D laboratories.
机译：值得注意的是，快速液相色谱（UFLC）和常规HPLC之间的线性方法转换在此应用中至关重要。在这项工作中观察到的良好线性关系可能与两个因素有关，相同类型的C18色谱柱（制造商，材料，填料和封盖技术等）以及在UFLC色谱柱和HPLC色谱柱的相同系统上。这些因素将色谱条件的差异降至最低，但填料的粒径和色谱柱尺寸除外。我们没有在不同类型的色谱柱和不同的系统上尝试过相同的过程。但是，可以预期的是，如果系统配置相同并且管路系统设置得当，可以最大程度地减少死角，则液相色谱系统的差异可能会导致保留时间的系统偏移，但不会影响快速液相色谱和常规HPLC之间的线性关系。卷。它没有检查不同制造商提供的列之间的线性方法转移关系。这项工作的结果表明，UFLC可以在HPLC方法开发中非常有用，以提高效率和生产率。这种方法对化学，食品和制药等各个行业几乎都是有益的，而传统HPLC在其QA / QC和R＆D实验室中仍然占主导地位。

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