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首页> 外文期刊>Acta Oceanologica Sinica >Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region
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Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region

机译：使用ITS区域的DNA分子标记鉴定菲律宾马尼拉蛤中Perkinsus样寄生虫

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摘要

Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China. The internal transcribed spacer(ITS) in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank. The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA(51 bp) and ITS(+5.8 S) (598 bp) regions. The ITS(+5.8S) sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those of Perkinsus atlanticus,and were not more than 95% identical to those of other four Perkinsus species including P. marinus, P. andrewsi, P. qugwadi and P. medierraneus .The ITS (+5.8S) sequence of Perkins us -like parasite of Shicheng Island was 99% identical to that of Ningbo. These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P. atlanticus, and might reflect P. atlanticus strains of distinct geographic distribution.
机译：基因组DNA是从收集于黄海沿岸石城岛和东海沿岸宁波渔场的马尼拉蛤仔Ruditapes philippinarum的Perkinsus样寄生虫的催芽孢子中提取的。对rDNA中的内部转录间隔子（ITS）进行PCR扩增，克隆，测序，并与GenBank中的五个Perkinsus物种进行比较。从石城岛或宁波寄生虫的DNA扩增的片段含有649 bp，包括部分ssrRNA（51 bp）和ITS（+5.8 S）（598 bp）区域。石城岛和宁波的Perkinsus样寄生虫的ITS（+ 5.8S）序列与Perkinsus atlanticus的ITS序列均具有99％的同源性，与包括P. marinus在内的其他4种Perkinsus物种的ITS（+ 5.8S）同源性不超过95％。 P. andrewsi，P。qugwadi和P. medierraneus。石城岛的Perkins us样寄生虫的ITS（+ 5.8S）序列与宁波的99％相同。这些有关核苷酸序列的事实表明，从黄海沿岸或东海沿岸收集的马尼拉蛤，菲律宾蛤仔中的珀金索斯样寄生虫是大西洋疟原虫，可能反映了地理分布不同的大西洋疟原虫菌株。

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