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首页> 外文期刊>Theoretical and Applied Genetics >RAPD-based SCAR marker SCA 12 linked to recessive gene conferring resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench]
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RAPD-based SCAR marker SCA 12 linked to recessive gene conferring resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench]

机译：基于RAPD的SCAR标记SCA 12与隐性基因相关联，可赋予对高粱炭疽病的抗性[Sorghum bicolor（L.）Moench]

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摘要

Anthracnose, caused by Colletotrichum graminicola, infects all aerial parts of sorghum, Sorghum bicolor (L.) Moench, plants and causes loss of as much as 70%. F1 and F2 plants inoculated with local isolates of C. graminicola indicated that resistance to anthracnose in sorghum accession G 73 segregated as a recessive trait in a cross with susceptible cultivar HC 136. To facilitate the use of marker-assisted selection in sorghum breeding programs, a PCR-based specific sequence characterized amplified region (SCAR) marker was developed. A total of 29 resistant and 20 susceptible recombinant inbred lines (RILs) derived from a HC 136 × G 73 cross was used for bulked segregant analysis to identify a RAPD marker closely linked to a gene for resistance to anthracnose. The polymorphism between the parents HC 136 and G 73 was evaluated using 84 random sequence decamer primers. Among these, only 24 primers generated polymorphism. On bulked segregant analysis, primer OPA 12 amplified a unique band of 383 bp only in the resistant parent G 73 and resistant bulk. Segregation analysis of individual RILs showed the marker OPA 12383 was 6.03 cM from the locus governing resistance to anthracnose. The marker OPA 12383 was cloned and sequenced. Based on the sequence of cloned RAPD product, a pair of SCAR markers SCA 12-1 and SCA 12-2 was designed using the MacVector program, which specifically amplified this RAPD fragment in resistant parent G 73, resistant bulk and respective RILs. Therefore, it was confirmed that SCAR marker SCA 12 is at the same locus as RAPD marker OPA 12383 and hence, is linked to the gene for resistance to anthracnose.
机译：炭疽菌（Colletotrichum graminicola）引起的炭疽病会感染高粱，双色高粱（L.）Moench，植物的所有地上部分，并造成多达70％的损失。接种了地方分枝梭状芽胞杆菌的F1 和F2 植物表明，高粱登录号G 73中对炭疽病的抗性在与易感品种HC 136的杂交中分离为隐性性状。在高粱育种程序的辅助选择中，开发了一种基于PCR的特异序列特征区域（SCAR）标记。来源于HC 136×G 73杂交的29株抗性和20株易感的重组自交系（RIL）用于大量分离物分析，以鉴定与炭疽病抗性基因紧密相连的RAPD标记。 HC 136和G 73亲本之间的多态性使用84个随机序列decamer引物进行了评估。其中，只有24个引物产生多态性。在大量分离物分析中，引物OPA 12仅在抗性亲本G 73和抗性主体中扩增了383 bp的独特条带。单个RILs的分离分析表明，标记OPA 12383 与控制炭疽病的抗性位点相距6.03 cM。标记OPA 12383 被克隆并测序。根据克隆的RAPD产物的序列，使用MacVector程序设计了一对SCAR标记SCA 12-1和SCA 12-2，它们在抗性亲本G 73，抗性体和相应的RIL中特异性扩增了该RAPD片段。因此，证实了SCAR标志物SCA 12与RAPD标志物OPA 12383 位于相同的位点，因此与抗炭疽病基因相连。

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  • 来源
    《Theoretical and Applied Genetics》 |2006年第1期|187-192|共6页
  • 作者

    Monika Singh; K. Chaudhary; K. S. Boora;

  • 作者单位

    Department of Biotechnology and Molecular Biology CCS Haryana Agricultural University;

    Department of Biotechnology and Molecular Biology CCS Haryana Agricultural University;

    Department of Biotechnology and Molecular Biology CCS Haryana Agricultural University;

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