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首页> 外文期刊>Indian Journal of Experimental Biology >RAPD based DNA markers linked to anthracnose disease resistance in Sorghum bicolor (L.) Moench.
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RAPD based DNA markers linked to anthracnose disease resistance in Sorghum bicolor (L.) Moench.

机译：基于RAPD的DNA标记与双色高粱（L.）Moench中的炭疽病抗性相关。

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摘要

Anthracnose caused by Colletotrichum graminicola is one of the major diseases of sorghum. The locus for disease resistance in sorghum [Sorghum biocolor (L.) Moench] accession G73 was found to segregate as a simple recessive trait in a cross to susceptible cultivar HC136. In order to identify molecular markers linked to the locus for disease resistance, random amplified polymorphic DNA (RAPD) analysis was coupled with bulk segregant analysis. DNA from the parental cultivars and the bulks were screened by PCR amplification with 114 RAPD primers. Three RAPD primers amplified a sequence that consegregated with the recessive resistance allele, while another three amplified a band linked to the susceptible allele. The six disease linked markers were screened with individual resistant and susceptible genotypes to observe degree of linkage of identified RAPD markers with the gene for resistance. Two primer sequences (OPI 16 and OPD 12) were found to be closely linked to the locus for disease resistance.
机译：炭疽菌引起的炭疽病是高粱的主要疾病之一。发现高粱[Sorghum biocolor（L.）Moench]登录号G73中的抗病性位点在与易感品种HC136的杂交中作为简单的隐性性状分离。为了鉴定与抗病性位点相关的分子标记，将随机扩增多态性DNA（RAPD）分析与本体分离子分析结合使用。用114个RAPD引物进行PCR扩增，筛选出来自亲本品种和大部分的DNA。三个RAPD引物扩增了与隐性抗性等位基因聚合的序列，另外三个扩增了与易感等位基因连接的条带。用个体抗药性和易感基因型筛选了六个疾病相关标记，观察已鉴定的RAPD标记与抗性基因的连锁程度。发现两个引物序列（OPI 16和OPD 12）与抗病性基因座紧密相连。

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