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Evaluation of the Appropriate Diagnostic Tools for Intra-Mammary Infection in Lactating Dairy Goats

机译:乳山羊乳内感染的适当诊断工具的评价

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Sixty milk samples of healthy, lactating dairy goats were collected by an aseptic, hand milking technique. All samples were analyzed using milk quality tests: bacterial isolation (Bac), total plate count (TPC), dye reduction test (DRT) and clot-on-boiling test (COB), as well as screening tests: the Californian mastitis test (CMT) and electrical resistance (ER), and somatic cell count (SCC) and percentage of milk composition including fat, protein, lactose and solid-not-fat (SNF). According to 34 samples for infra-mammary infection (IMI) and 26 samples for non-IMI, the results showed that Bac and TPC were the most reliable techniques for the determination of IMI with strong correlations. For an instantaneous milk quality test, CMT reaction was probably more reliable as there was a statistical significance between bacteriologically positive and negative samples (P = 0.06). Moreover, CMT showed a correlation with TPC, Bac, DRT, ER, ER ref, SCC, milk protein, and milk lactose (P ≤ 0.05). Based on the range in ER, TPC showed a statistically significant difference between the ER level being less than 350 units and higher than 400 units (P ≤ 0.05). Except for CMT reactions, SCC did not correlate to other measurements and did not indicate the IMI status of goats. These findings indicated that there was a need for future improvement in the diagnostic tools for IMI in goats.
机译:通过无菌手工挤奶技术收集了六十只健康的,泌乳期的山羊乳样品。使用牛奶质量测试对所有样品进行分析:细菌分离(Bac),总板数(TPC),染料还原测试(DRT)和沸腾凝块测试(COB),以及筛查测试:加利福尼亚乳腺炎测试( CMT)和电阻(ER),体细胞计数(SCC)以及乳成分的百分比,包括脂肪,蛋白质,乳糖和非脂肪固体(SNF)。根据34个乳腺感染(IMI)样本和26个非IMI样本,结果表明Bac和TPC是确定IMI且相关性最强的最可靠技术。对于瞬时牛奶质量测试,CMT反应可能更可靠,因为细菌学阳性和阴性样品之间存在统计学意义(P = 0.06)。此外,CMT与TPC,Bac,DRT,ER,ER ref,SCC,乳蛋白和乳乳糖相关(P≤0.05)。根据ER的范围,TPC显示ER水平小于350单位和大于400单位之间存在统计学上的显着差异(P≤0.05)。除CMT反应外,SCC与其他测量值无关,也未显示山羊的IMI状态。这些发现表明,有必要对山羊IMI的诊断工具进行进一步的改进。

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