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首页> 外文期刊>The Kasetsart Journal >Observation of Two Viruses in Cultured Giant Freshwater Prawns (Macrobrachium rosenbergii de Man) in Thailand
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Observation of Two Viruses in Cultured Giant Freshwater Prawns (Macrobrachium rosenbergii de Man) in Thailand

机译：在泰国养殖的淡水虾（Macrobrachium rosenbergii de Man）中观察到两种病毒

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摘要

Two viruses in the hepatopancreas (HP) of Macrobrachium rosenbergii postlavae (PL) and adult prawns are described. Investigations were carried out by light microscopic examination of HP tissue squashes stained with 0.05 % malachite green and histological sections stained with hematoxylin and eosin (H&E), as well as by transmission electron microscopy (TEM). Histologically, the first pathogen was a baculo-like virus (BLV), recognized by the presence of enlarged nuclei with marginated nucleoli and eosinophilic inclusions that completely filled the nucleus of the HP tubular epithelium. In contrast, the malachite green-stained HP squashes did not show any marginated nucleoli. TEM of the infected HPs revealed the presence of BLV particles of approximately 250-300 nm in enlarged nuclei. The second virus was a hepatopancreatic parvo-like virus (HPLV). Spherical intranuclear inclusions were easily observed in the HP tubular epithelium in both fresh malachite green-stained tissue squashes and H&E-stained histological sections. TEM of infected HPs revealed intranuclear inclusions containing small virus-like particles of approximately 20-24 nm in diameter.rnPL (n=200) with percentage infection rates of 20% with the BLV, 50% with the HPLV and 10% with dual infection were stocked into earthen ponds at a density of 60 000 PL/1600 m~2. After rearing for 60 d, the prawns were transferred into earthen ponds at a density of 10 000 prawns/1600 m~2. Sixty days later, (120-day-old) prawns were transferred into earthen ponds at a density of 5000 prawns/ 1600 m~2 and reared until harvesting at day 180. The prawns were sampled and divided into small and large-sized prawns at 60, 120, 150 and 180 d for histological studies to determine the viral infections. Results showed that at 120 d, the BLV infection in the small-sized prawns (12.5%) was higher than that of the large-sized prawns (5%). Dual infection with both viruses was 5.0% in small-sized prawns and 2.5% in large-sized prawns. However, at day 150, the BLV pathogen was not observed in either size group. In contrast, the HPLV infection was found in prawns throughout the culture period. The percentage infection rates in small-sized prawns at days 120,150 and 180 were 22.5,15.0 and 12.5%, respectively, similar to the infection rates for large-sized prawns of 20.0,12.5 and 12.5%, respectively. The percentage survival rate and yield from both ponds at harvest were 67-68% and 197-199 kg/1600 m~2, respectively. These results indicated that the infection rates of these two viruses did not increase during the culture period and that the virus infections might have a negative effect on production.
机译：描述了罗氏沼虾（PL）和成年虾的肝胰腺（HP）中的两种病毒。通过光学显微镜检查用0.05％孔雀石绿染色的HP组织南瓜和用苏木精和曙红（H＆E）染色的组织学切片以及通过透射电子显微镜（TEM）进行研究。从组织学上讲，第一个病原体是杆状病毒（BLV），被存在核仁增大和嗜酸性包涵体的核增大而识别，该夹杂物完全充满了HP肾小管上皮细胞核。相反，孔雀石绿染色的HP南瓜没有显示出边缘化的核仁。感染的HP的TEM显示出在扩大的核中存在约250-300nm的BLV颗粒。第二种病毒是肝胰细小病毒样病毒（HPLV）。在新鲜的孔雀石绿染色的组织南瓜和H＆E染色的组织切片中，在HP管状上皮中均易于观察到球形核内包裹体。感染的HP的TEM显示核内夹杂物包含直径约20-24 nm的小病毒样颗粒.rnPL（n = 200），BLV的感染率为20％，HPLV的感染率为50％，双重感染的感染率为10％被放到密度为60 000 PL / 1600 m〜2的土池中。养育60天后，将虾以10000只大虾/ 1600 m〜2的密度转移到土塘中。六十天后，将（120天龄）对虾以5000对虾/ 1600 m〜2的密度转移到土池中，并进行养育，直到180天收获为止。对虾进行采样，并按大小将其分为大小对虾60、120、150和180 d进行组织学研究以确定病毒感染。结果表明，在120 d时，小型虾的BLV感染（12.5％）高于大型虾的BLV感染（5％）。两种病毒的双重感染在小型虾中分别为5.0％和在大型虾中为2.5％。然而，在第150天，在任一大小组中均未观察到BLV病原体。相反，在整个培养期间，在虾中发现了HPLV感染。在120,150天和180天，小型虾的感染率分别为22.5、15.0和12.5％，与大型虾的感染率分别为20.0、12.5和12.5％。收获时两个池塘的成活率和产量分别为67-68％和197-199 kg / 1600 m〜2。这些结果表明在培养期间这两种病毒的感染率没有增加，并且该病毒感染可能对生产有负面影响。

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