• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Kasetsart Journal >Characterization of Antibacterial Protein from Hemolymph of Oyster Crassostrea belcheri
【24h】

Characterization of Antibacterial Protein from Hemolymph of Oyster Crassostrea belcheri

机译：牡蛎Crassostrea belcheri血淋巴中抗菌蛋白的表征

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The hemolymph of oyster Crassostrea belcheri was found to possess significant antibacterial activity against Vibrio harveyi, V. vulnificus and V. cholerae. Hemolymph was partially purified by gel filtration chromatography on Sephacryl S-200 and fractioned into four parts: P1, P2, P3 and P4. The P3 showed the highest antibacterial activity against V. parahaemolyticus, V. cholerae, V. alginolyticus, V. harveyi in the inhibition ranges from 95-62% and V. vulnificus was inhibited only 8% with 64 mg protein. The antibacterial activity of P3 protein was stabilized at pH 6-8 and 4-30℃. In addition, the activity required calcium ion. Molecular weight of the antibacterial protein P3 determined by gel filtration was approximately 18.7 kDa. Antibacterial protein gave two bands of 25.0 and 30.5 kDa in SDS-PAGE and pI was 3.0 and 5.0, respectively. Both bands were analyzed by LC-MS/MS and compared with nrFasta database. The 30.5 kDa band was homologous to hemocyte extracellular superoxide dismutase from C. gigas and 25.0 kDa band was homologous to sarcoplasmic calcium-binding protein.
机译：发现牡蛎Crassostrea belcheri的血淋巴对哈维弧菌，创伤弧菌和霍乱弧菌具有显着的抗菌活性。血淋巴通过在Sephacryl S-200上的凝胶过滤色谱法进行部分纯化，并分为四个部分：P1，P2，P3和P4。 P3对溶血弧菌，霍乱弧菌，溶藻弧菌，哈维氏弧菌显示出最高的抗菌活性，其抑制作用范围为95-62％，而用64 mg蛋白仅能抑制V. vulnificus 8％。在pH 6-8和4-30℃下，P3蛋白的抗菌活性稳定。另外，该活性需要钙离子。通过凝胶过滤测定的抗菌蛋白P3的分子量约为18.7kDa。抗菌蛋白在SDS-PAGE中显示两个条带分别为25.0和30.5 kDa，pI分别为3.0和5.0。通过LC-MS / MS分析两个频带，并与nrFasta数据库进行比较。 30.5kDa条带与来自C. gigas的血细胞胞外超氧化物歧化酶同源，而25.0kDa条带与肌浆钙结合蛋白同源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号