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首页> 外文期刊>Aquaculture >Non-lethal determination of sex and reproductive condition of Eastern oysters Crassostrea virginica Gmelin using protein profiles of hemolymph by ProteinchipA registered and SELDI-TOF-MS technology
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Non-lethal determination of sex and reproductive condition of Eastern oysters Crassostrea virginica Gmelin using protein profiles of hemolymph by ProteinchipA registered and SELDI-TOF-MS technology

机译：通过ProteinchipA注册和SELDI-TOF-MS技术使用血淋巴的蛋白质谱非致命性测定东部牡蛎Crassostrea virginica Gmelin的性别和生殖状况

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As the demand for hatchery-produced oyster seed increases for both aquaculture and restoration purposes, techniques to improve seed production, such as accurate determination of broodstock oyster sex and gonad development, have become more important. In this study, we developed a novel method of determining oyster sex and developmental stage through protein profiling of hemolymph using a relatively new proteomic tool, ProteinchipA registered and SELDI-TOF-MS. Over 139 peptides/proteins were detected from oyster hemolymph, 62 of which appeared to be involved in reproductive activities. Using the protein-profile information, individual broodstock oysters were categorized successfully into one of five groups: undifferentiated, female developing, female ripe, male developing and male ripe. The accuracy of categorization, confirmed by subsequent, traditional histological methods, was 98.8% (p <0.05). Wide application of this method is still limited by cost; however, results of this research open doors for further study to develop more-affordable and portable methods based upon detection of specific hemolymph peptides and proteins.
机译：随着用于水产养殖和恢复目的对孵化场生产的牡蛎种子的需求增加，用于改善种子产量的技术（例如准确确定亲鱼牡蛎性别和生殖腺发育）变得越来越重要。在这项研究中，我们开发了一种通过使用相对较新的蛋白质组学工具，ProteinchipA注册和SELDI-TOF-MS对血淋巴进行蛋白谱分析来确定牡蛎性别和发育阶段的新方法。从牡蛎血淋巴中检测到了139种以上的肽/蛋白质，其中62种似乎与生殖活动有关。利用蛋白质谱信息，将亲鱼牡蛎成功地分为五组之一：未分化，雌性发育，雌性成熟，雄性发育和雄性成熟。随后的传统组织学方法证实了分类的准确性为98.8％（p <0.05）。这种方法的广泛应用仍然受到成本的限制。然而，这项研究的结果为进一步研究以检测特定的血淋巴肽和蛋白质为基础，开发出负担得起的便携式方法打开了大门。

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