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首页> 外文期刊>The Kasetsart Journal >Anti HSV-1 Activity of Spirulina platensis Polysaccharide
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Anti HSV-1 Activity of Spirulina platensis Polysaccharide

机译：螺旋藻多糖的抗HSV-1活性

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摘要

Aqueous extracts of Spirulina platensis were precipitated by cetyltrimethylammonium bromide (CTAB). The hot water extract was found anti Herpes simplex virus type 1 (HSV-1) activity at 50% inhibitory concentration (IC_(50)) values of 21.32 μg/ml. Partial purification by gel filtration of the crude extract on Sepharose 6B column gave two fractions, SHP-F1 and SHP-F2, which revealed about 4 and 2 times higher activity than that of the crude hot water extract, respectively. The crude hot water extract was a polysaccharide with rhamnose as the main sugar component. Calcium ion and sulfate groups in this polysaccharide had major roles in antiviral activity. However, the crude hot water extract polysaccharide contained approximately 42% carbohydrate and 31% protein. Decreasing the amount of protein by precipitation with trichloroacetic acid (TCA) resulted in higher purity of the crude hot water extract polysaccharide.
机译：十六烷基三甲基溴化铵（CTAB）沉淀螺旋藻的水提物。发现该热水提取物在21.32μg/ ml的50％抑制浓度（IC_（50））值下具有抗1型单纯疱疹病毒（HSV-1）的活性。在Sepharose 6B色谱柱上通过凝胶过滤粗提物进行部分纯化，得到两个馏分SHP-F1和SHP-F2，分别显示出比粗热水提取物高约4倍和2倍的活性。粗热水提取物是一种以鼠李糖为主要糖成分的多糖。该多糖中的钙离子和硫酸根基团在抗病毒活性中起主要作用。但是，粗热水提取物多糖包含大约42％的碳水化合物和31％的蛋白质。通过用三氯乙酸（TCA）沉淀来减少蛋白质的量，可以得到较高纯度的热水提取物多糖。

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