...
首页> 外文期刊>The Kasetsart Journal >Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay
【24h】

Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay

机译:酶联免疫吸附法定量测定饲料中O曲毒素A的单克隆抗体生产

获取原文
获取原文并翻译 | 示例
           

摘要

Ochratoxin A (OTA), a mycotoxin, is produced from Aspergillus and Penicillium species. It is a nephrotoxin which is very toxic for the kidneys, and normally found in feedstuffs and other feeds. In this study, the monoclonal antibody (MAb) against OTA was produced to determine OTA in feedstuffs. It was found that 3 monoclones (5E7-3F2, 5E7-3G6 and 5E7-G10) reacted with OTA. MAb developed a specific and highly sensitive sandwich ELISA. No difference in quantification in samples was found when compared with a commercial test kit (P > 0.05) and 50% binding of standard curve was found at 35 pg/assay. In this study OTA in feedstuff could be detected by MAb, which was self-producing by the ELISA method.
机译:曲霉毒素A(OTA)是一种霉菌毒素,由曲霉和青霉菌产生。它是一种对肾脏有毒的肾毒素,通常存在于饲料和其他饲料中。在这项研究中,产生了针对OTA的单克隆抗体(MAb),以确定饲料中的OTA。发现3种单克隆(5E7-3F2、5E7-3G6和5E7-G10)与OTA反应。 MAb开发了一种特异性高灵敏度的夹心ELISA。与商用测试试剂盒相比,样品的定量无差异(P> 0.05),在35 pg /次分析中发现50%的标准曲线结合。在这项研究中,饲料中的OTA可以通过单克隆抗体(MAb)进行检测,而单克隆抗体是通过ELISA方法自行产生的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号