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首页> 外文期刊>The Kasetsart Journal >Production of In-house ELISA Test Kit for Detection of Aflatoxin in Agricultural Commodities and Their Validations
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Production of In-house ELISA Test Kit for Detection of Aflatoxin in Agricultural Commodities and Their Validations

机译：用于农产品中黄曲霉毒素检测的内部ELISA测试试剂盒的生产及其验证

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A specific microtest plate enzyme immunoassay was developed in Thailand for the rapid detection of aflatoxinB_1 (AFB_1)using Poly clonal antibody against AFB_1 Percentage of the antibody cross reaction to AFB_1, AFB_2, AFG_1 and AFG_2 were 100, 21.4, 25.0 and 2.5% respectively. AFB_1HRP (Horseradish Peroxidase) conjugate was used as direct competitive test for AFB_1 detection. The DO A- Aflatoxin ELISA Test Kit was produced for detection of both qualitative and quantitative results within 1 hr after sample preparation with the minimum of 0.4ppbper assay. There was no significant difference in detection efficiency of this test kit compared to traditional methods of minicolumn, TLC and HPLC. Moreover, the kit obtained 82-100% AFB_1 recovery from the spike samples. Validation for the analysis of AFB_1 in sample matrices was also tested using peanut extract which had the virtually identical curves to the standard. Detection efficiency of DOA-Aflatoxin ELISA test kit was not different from those imported from the USA and Italy.
机译：在泰国开发了一种特异性微量酶标板酶免疫测定法，用于使用针对AFB_1的多克隆抗体快速检测黄曲霉毒素B_1（AFB_1）。与AFB_1，AFB_2，AFG_1和AFG_2发生交叉反应的百分比分别为100％，21.4、25.0和2.5％。 AFB_1HRP（辣根过氧化物酶）结合物被用作AFB_1检测的直接竞争测试。生产DO A-黄曲霉毒素ELISA测试试剂盒，用于在样品制备后1小时内以最小0.4ppbper测定法检测定性和定量结果。与传统的微型柱，TLC和HPLC方法相比，该试剂盒的检测效率没有显着差异。此外，该试剂盒从加标样品中回收了82-100％的AFB_1。还使用花生提取物测试了样品基质中AFB_1的分析验证，该提取物具有与标准品几乎相同的曲线。 DOA-黄曲霉毒素ELISA检测试剂盒的检测效率与从美国和意大利进口的检测效率没有区别。

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