...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Translational Stroke Research >Prolonged Exposure to Isoflurane Ameliorates Infarction Severity in the Rat Pup Model of Neonatal Hypoxia-Ischemia
【24h】

Prolonged Exposure to Isoflurane Ameliorates Infarction Severity in the Rat Pup Model of Neonatal Hypoxia-Ischemia

机译：长期暴露于异氟烷可减轻新生鼠缺氧缺血大鼠模型的梗死严重程度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The neonatal hypoxia-ischemia rat model referred to as the Rice–Vannucci model is extensively used to study perinatal hypoxia-ischemia and child brain injury. One of the major weaknesses of this model is its inconsistency of brain infarction among animals. We hypothesize that the inconsistency of infarction is caused by prolonged operation time and therefore isoflurane exposure. Neonatal hypoxia-ischemia was induced in postnatal days 7 and 10 rat pups by unilateral right common carotid ligation followed by 2.5 h of hypoxia (8% oxygen). The incision-to-ligation (ITL) was defined as the amount of time from initial incision (4 min after 2% isoflurane exposure) to completion of carotid ligation (at which point isoflurane exposure was also terminated). In the first part of the study, the ITL of each group was designated to be 5, 13, and 21 min. In the second part of the study, the ITL is designated to 4 min; however, continued isoflurane was used to make 5, 13, and 21 min isoflurane exposure for each group. Percentages of brain infarction were assessed at 48 h following surgery. Motor deficits were accessed by Rotarod test. Marked brain infarction was observed in the 5-min ITL group and a decrease of brain infarction observed in the 13- and 21-min groups (P < 0.05). In the second part of the study, marked brain infarction was observed in the 5-min isoflurane exposure group, and a decrease of brain infarction was observed in each of the 13- and 21-min isoflurane exposure groups (P < 0.05). Similar tendencies were observed in Rotarod tests than 5-min ITL and 5-min isoflurane groups showed more marked deficits (P < 0.05). This study demonstrated that brain infarction inconsistency of the neonatal hypoxia-ischemia rat pup model is related to the operation time. The observed time-dependent decrease of brain infarction is correlated to the isoflurane exposure time. Shorter operation and isoflurane exposure improves this model consistency of brain infarction and motor deficits.
机译：新生婴儿缺氧缺血大鼠模型被称为Rice–Vannucci模型，被广泛用于研究围产期缺氧缺血和儿童脑损伤。该模型的主要缺点之一是动物间脑梗塞的不一致。我们假设梗塞的不一致是由于手术时间延长和异氟烷暴露引起的。在出生后的第7天和第10天，通过单侧右颈总动脉结扎，然后进行2.5小时的缺氧（8％的氧气）诱导新生的新生儿缺氧缺血。切口结扎（ITL）定义为从初次切口（2％异氟烷暴露后4分钟）到完成颈动脉结扎（此时异氟烷暴露也终止）的时间。在研究的第一部分中，将每组的ITL指定为5、13和21分钟。在研究的第二部分中，将ITL指定为4分钟。但是，每组分别使用持续的异氟烷暴露5、13和21分钟。在手术后48小时评估脑梗死的百分比。运动缺陷通过罗塔罗德检验获得。在5分钟的ITL组中观察到明显的脑梗塞，在13分钟和21分钟的组中观察到脑梗塞的减少（P <0.05）。在研究的第二部分中，在5分钟的异氟烷暴露组中观察到明显的脑梗塞，在13分钟和21分钟的异氟烷暴露组中均观察到脑梗塞的减少（P <0.05）。与5分钟ITL相比，在Rotarod测试中观察到了相似的趋势，而5分钟异氟烷组显示出更明显的缺陷（P <0.05）。这项研究表明，新生儿缺氧缺血大鼠幼鼠模型的脑梗死不一致与手术时间有关。观察到的时间依赖性的脑梗塞减少与异氟烷暴露时间有关。较短的手术时间和异氟烷暴露可改善这种模型的脑梗死和运动障碍的一致性。

著录项

  • 来源
    《Translational Stroke Research》 |2011年第3期|382-390|共9页
  • 作者

    Hank Chen; Michael Burris; Adrain Fajilan; Fred Spagnoli; John H. Zhang; Jiping Tang;

  • 作者单位

    Department of Physiology and Pharmacology Loma Linda University School of Medicine Loma Linda CA 92350 USA;

    Department of Physiology and Pharmacology Loma Linda University School of Medicine Loma Linda CA 92350 USA;

    Department of Physiology and Pharmacology Loma Linda University School of Medicine Loma Linda CA 92350 USA;

    Department of Physiology and Pharmacology Loma Linda University School of Medicine Loma Linda CA 92350 USA;

    Department of Physiology and Pharmacology Loma Linda University School of Medicine Loma Linda CA 92350 USA;

    Department of Physiology and Pharmacology Loma Linda University School of Medicine Loma Linda CA 92350 USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Neonatal hypoxia-ischemia; Isoflurane; Neuroprotection; Rotarod test;

    机译：新生儿缺氧缺血;异氟烷;神经保护;Rotarod试验;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号