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首页> 外文期刊>Tropical Animal Health and Production >Serological and bacteriological responses of water buffalo (Bubalus bubalis) vaccinated with two doses of Brucella abortus strain RB51 vaccine
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Serological and bacteriological responses of water buffalo (Bubalus bubalis) vaccinated with two doses of Brucella abortus strain RB51 vaccine

机译：接种两剂布鲁氏菌流产RB51疫苗的水牛（Bubalus bubalis）的血清学和细菌学应答

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摘要

Thirty-two water buffalo (Bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to Brucella abortus strain RB51 (RB51) vaccination in a dose–response study and to compare the use of two selective media for the isolation of RB51. The animals were randomly divided into three treatment groups. Groups I–III received the recommended vaccine dose (RD) twice 4 weeks apart, RD twice 18 weeks apart and saline once, respectively. Lymph nodes were excised from the three groups and subjected to bacteriological examination to determine the frequency of detection of RB51. Pre- and post-vaccination blood samples were collected and tested for B. abortus antibodies using the buffered plate agglutination test (BPAT), complement fixation test (CFT), and dot-blot assay. Sera taken at all post-inoculation weeks (PIW) were negative for field strain B. abortus using the BPAT. Antibody responses to RB51 were demonstrated in all vaccinates but not in controls by CFT and dot-blot assay from 1 PIW up to 16 weeks following booster vaccination. The agreement for both assays was 80.7% and there was a linear interdependence with a Pearson’s correlation coefficient value of 0.578. The frequency of isolation of RB51 from the two selective media used was not significantly different (P > 0.05).
机译：在剂量-反应研究中，使用32只6-10个月大的水牛（Bubalus bubalis）小牛来评估对流产布鲁氏菌RB51（RB51）疫苗的血清学反应，并比较使用两种选择性培养基分离RB51的情况。 。将动物随机分为三个治疗组。 I–III组分别接受间隔4周两次，间隔18周两次RD和一次生理盐水的推荐疫苗剂量（RD）。从三组中切除淋巴结，并进行细菌学检查以确定RB51的检测频率。收集疫苗接种前后的血样，并使用缓冲板凝集试验（BPAT），补体固定试验（CFT）和斑点印迹法测试流产双歧杆菌抗体。在接种后的所有周（PIW）采集的血清，使用BPAT对田间菌株B.流产呈阴性。通过加强免疫接种后的1个PIW至16周内的CFT和斑点印迹分析，在所有疫苗中均显示出对RB51的抗体反应，但未在对照中显示。两种检测方法的一致性为80.7％，线性相关性为Pearson相关系数值为0.578。从使用的两种选择培养基中分离出RB51的频率没有显着差异（P> 0.05）。

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  • 来源
    《Tropical Animal Health and Production》 |2012年第7期|1451-1458|共8页
  • 作者

    Anil Ramnanan; Michael Diptee; Zinora Asgarali; Mervyn Campbell; Abiodun Adewale Adesiyun;

  • 作者单位

    Ministry of Agriculture Lands and Marine Resources St. Clair Port of Spain Trinidad and Tobago;

    School of Veterinary Medicine Faculty of Medical Sciences University of the West Indies Champs Fleurs St. Augustine Trinidad and Tobago;

    School of Veterinary Medicine Faculty of Medical Sciences University of the West Indies Champs Fleurs St. Augustine Trinidad and Tobago;

    School of Veterinary Medicine Faculty of Medical Sciences University of the West Indies Champs Fleurs St. Augustine Trinidad and Tobago;

    School of Veterinary Medicine Faculty of Medical Sciences University of the West Indies Champs Fleurs St. Augustine Trinidad and Tobago;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Brucella abortus; RB51; Vaccination; Water buffalo;

    机译：流产布鲁氏菌;RB51;疫苗接种;水牛;

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