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首页> 外文期刊>Veterinary Immunology and Immunopathology >Serologic responses, biosafety and clearance of four dosages of Brucella abortus strain RB51 in 6-10 months old water buffalo (Bubalus bubalis).
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Serologic responses, biosafety and clearance of four dosages of Brucella abortus strain RB51 in 6-10 months old water buffalo (Bubalus bubalis).

机译：在6-10个月大的水牛（Bubalus bubalis）中，四种剂量的流产布鲁氏菌RB51的血清学响应，生物安全性和清除率。

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Thirty water buffalo were obtained from a brucellosis-free farm in order to evaluate antibody responses, bacterial clearance and safety to Brucella abortus strain RB51 vaccine in a dose response study. The animals were randomly divided into five treatment groups. Groups I-V received the recommended dose of RB51 vaccine (RD) once, RD twice 4 weeks apart, double RD once, double RD twice 4 weeks apart and saline once, respectively. Antibody responses to RB51 were monitored at 2, 4, 6, 8, 10, 12, 16 18, 22, 24 and 27 post-initial-inoculation weeks (PIW). Clearance of RB51 from the prescapular lymph node was evaluated at 2, 4, 6, 12, 18 and 24 PIW for groups 1, III and V and at 6, 8, 10, 16, 22 and 27 PIW for groups II and IV. To evaluate shedding of the RB51 strain, nasal, conjunctival, vaginal or preputial swabs were taken from all experimental animals at 1, 2, 3, 4, 6, 8 and 12 PIW. Sera taken at all PIW were negative for field strain B. abortus by both the buffered plate agglutination test (BPAT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Antibody responses to RB51 were demonstrated in all vaccinates but not in the controls, up to 12 PIW, by complement fixation test (CFT) and the dot-blot assay with an 83.7% agreement for both tests. Clearance of RB51 occurred between 6 and 12 PIW in group I but less than 2 weeks after booster vaccinations in groups II and IV and between 4 and 6 PIW in group III. RB51 was not recovered at any time from swabs obtained from either RB51-vaccinates or non-vaccinates. The results of this study indicate that serologic responses to RB51 vaccination can be monitored by both CFT and dot-blot assay in water buffalo. Our data also indicates that RB51 vaccination does not interfere with brucellosis sero-surveillance and is safe (no serological and bacteriological evidence of spread to non-vaccinates, no adverse clinical signs or detectable abnormalities on haematology and serum biochemistry) for use in water buffalo..
机译：从无布鲁氏菌病的农场获得了30只水牛，以在剂量反应研究中评估抗体对抗体的反应，细菌清除率和对流产布鲁氏菌RB51疫苗的安全性。将动物随机分为五个治疗组。 I-V组分别接受推荐剂量的RB51疫苗（RD），RD间隔4周两次，两次RD两次，间隔4周两次RD，两次生理盐水。在接种后的第2周，第2、4、6、8、10、12、16、18、22、24和27周监测对RB51的抗体反应。对于第1，III和V组，在肩W前淋巴结清除RB51的清除率分别为2、4、6、12、18和24 PIW，对于第II和IV组，以6、8、10、16、22和27 PIW进行评估。为了评估RB51菌株的脱落，从1、2、3、4、6、8和12 PIW的所有实验动物中取出鼻，结膜，阴道或包皮拭子。通过缓冲板凝集试验（BPAT）和竞争性酶联免疫吸附测定（c-ELISA），所有PIW采集的血清均对田间菌株B.流产呈阴性。通过补体固定试验（CFT）和斑点印迹试验，在所有疫苗中（最多12个PIW）都证明了对RB51的抗体应答，两种试验的一致性为83.7％。 RB51的清除发生在I组的6至12个PIW之间，但在II和IV组的加强免疫后不到2周以及III组的4至6 PIW之间。 RB51在任何时候都无法从从RB51疫苗或非疫苗获得的拭子中回收。这项研究的结果表明，在水牛中，可以通过CFT和斑点印迹法监测对RB51疫苗接种的血清学反应。我们的数据还表明，RB51疫苗接种不会干扰布鲁氏菌病的血清监测，并且可以安全地用于水牛（没有传播到非疫苗的血清学和细菌学证据，没有不良的临床体征或血液学和血清生化异常）。 。

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