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首页> 外文期刊>Tumor Biology >Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma
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Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma

机译：人骨肉瘤和成骨细胞的质膜蛋白质组学分析：揭示NDRG1作为骨肉瘤的标志物

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摘要

Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. To identify new biomarkers for early diagnosis of OS and novel therapeutic candidates, we carried out a plasma membrane proteomic study based on two-dimensional electrophoresis (2DE). The OS cell line MG-63 and the human osteoblastic cell line hFOB1.19 were adopted as the comparison model. We extracted plasma membrane by aqueous two-phase partition extraction. The proteins were separated through 2DE. We analyzed the differentially expressed proteins by Imagemaster software and then identified them by liquid chromatography–tandem mass spectrometry, and the location and function of differential proteins were searched through the Gene Ontology database. In total, 220 protein spots were separated by 2DE. Seven proteins with more than 2.0-folds of difference were successfully identified from 13 gel spots, with 6 up-regulated and 1 down-regulated. Gene Ontology analysis of the differentially expressed proteins indicated that these proteins were involved in seven kinds of functions including binding, structural, cell motility, receptor activity, electron carrier activity, NADH dehydrogenase (ubiquinone) activity, and transcription repressor activity. The up-regulation of NDRG1 was verified in osteosarcoma through Western blotting and by immunohistochemistry in paraffin-embedded tissues. The plasma membrane proteins identified in this study may provide new insights into osteosarcoma cancer biology and potential diagnostic and therapeutic biomarkers.
机译：骨肉瘤（OS）是儿童和青少年中最常见的原发性恶性骨肿瘤。为了鉴定用于OS的早期诊断的新生物标记物和新型治疗候选物，我们基于二维电泳（2DE）进行了质膜蛋白质组学研究。将OS细胞系MG-63和人成骨细胞系hFOB1.19作为比较模型。我们通过水相两相分配萃取法提取质膜。通过2DE分离蛋白质。我们通过Imagemaster软件分析了差异表达的蛋白质，然后通过液相色谱-串联质谱法对其进行了鉴定，并通过基因本体数据库搜索了差异蛋白质的位置和功能。共有2DE分离了220个蛋白质斑点。从13个凝胶斑点中成功鉴定出7种差异超过2.0倍的蛋白质，其中6个上调和1个下调。对差异表达蛋白的基因本体分析表明，这些蛋白涉及七种功能，包括结合，结构，细胞运动，受体活性，电子载体活性，NADH脱氢酶（泛醌）活性和转录阻遏物活性。骨肉瘤中通过蛋白质印迹法和石蜡包埋组织的免疫组织化学证实了NDRG1的上调。在这项研究中鉴定的质膜蛋白可能为骨肉瘤癌生物学以及潜在的诊断和治疗生物标志物提供新的见解。

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  • 来源
    《Tumor Biology》 |2011年第5期|1013-1021|共9页
  • 作者

    Yingqi Hua; Xiaofang Jia; Mengxiong Sun; Longpo Zheng; Lin Yin; Lijun Zhang; Zhengdong Cai;

  • 作者单位

    Musculoskeletal Oncology Center Shanghai 10th People’s Hospital Tongji University School of Medicine Shanghai 200072 China;

    Shanghai Public Health Clinical Center affiliated to Fudan University Shanghai 201508 China;

    Musculoskeletal Oncology Center Shanghai 10th People’s Hospital Tongji University School of Medicine Shanghai 200072 China;

    Musculoskeletal Oncology Center Shanghai 10th People’s Hospital Tongji University School of Medicine Shanghai 200072 China;

    Shanghai Public Health Clinical Center affiliated to Fudan University Shanghai 201508 China;

    Shanghai Public Health Clinical Center affiliated to Fudan University Shanghai 201508 China;

    Musculoskeletal Oncology Center Shanghai 10th People’s Hospital Tongji University School of Medicine Shanghai 200072 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Osteosarcoma; Plasma membrane; Proteomics; NDRG1; 2DE;

    机译：骨肉瘤;等离子膜;蛋白质组学;NDRG1;2DE;

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