Encephalomyocarditis (EMC) virus was studied for ability to agglutinate erythrocytes of various species. Human, rat, and guinea pig erythrocytes, as well as those from young rabbits, were readily agglutinated. Cells from older rabbits absorbed virus poorly, and showed little agglutination. Uptake of virus by rabbit brain also diminished with age. Various mouse tissues absorbed virus about equally well. Hemagglutination-inhibition studies demonstrated that a number of simple sugars, particularly glucose and galactosamine, interfered with uptake of virus by cells. Dextran sulfates were highly active inhibitors of EMC hemagglutination. Attempts to localize the site of action of the sugars on virus or cell are described. Treatment of virus with periodate or p-chloromercurobenzoate, and acetylation of virus, inhibited hemagglutination, but acetylation of semipurified receptor did not. Clarification of the nature of the virus-cell union will require studies to identify possible specific sugars in the virus capsid and the cell receptor.
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机译：研究了脑膜炎（EMC）病毒，用于粘结各种物种的红细胞的能力。人，大鼠和豚鼠红细胞以及幼兔子的人，易于凝集。来自较旧的兔子的细胞吸收了病毒差，并且表现出很少的凝集。兔脑吸收病毒也随着年龄的增长而减少。各种小鼠组织吸收了同样良好的病毒。血凝抑制研究表明，许多简单的糖，特别是葡萄糖和半乳糖胺，干扰了细胞的吸收。硫酸葡聚糖是EMC血凝的高活性抑制剂。描述了对病毒或细胞进行糖的作用遗址的尝试。治疗病毒与周期或 p -Chloromcurobenate，病毒的乙酰化，抑制血凝，但是精神化受体的乙酰化没有。澄清病毒细胞联盟的性质将需要研究以识别病毒衣壳和细胞受体中可能的特异性糖。
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