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首页> 外文期刊>Journal of Virology >Temperature-Sensitive Simian Virus 40-Transformed Cells: Phenomena Accompanying Transition from the Transformed to the “Normal” State
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Temperature-Sensitive Simian Virus 40-Transformed Cells: Phenomena Accompanying Transition from the Transformed to the “Normal” State

机译：温度敏感的Simian病毒40转化细胞：从转变转变为“正常”状态的过渡现象

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摘要

Temperature-sensitive simian virus (SV 40)-transformed 3T3 cells (tsSV3T3), which express the transformed phenotype when growing at 32 C but not at 39 C, were used to study changes in growth behavior during shift-up or shift-down experiments. In cultures of tsSV3T3 cells which had reached or were beyond monolayer density at 32 C, DNA synthesis reached very low levels within 24 to 48 h after shift-up. When cells which had been allowed to grow to high densities at 32 C were shifted to 39 C, not only cell growth stopped, but within two to three days the cultures shed a large number of cells into the medium. These cells were nonviable, and shedding stopped only when the number of cells attached had been reduced to that characteristic of the saturation density at 39 C. The remaining attached cells were viable and after the shift to 32 C were again able to grow from the monolayer to high cell densities. This behavior has been compared with that of normal 3T3 and wild-type SV3T3 cells under different conditions. We have also isolated new tsSV3T3 lines, using cells which had been infected with non-mutagenized wild-type SV40. This further demonstrates that the temperature sensitivity of these lines is due to a cellular rather than a viral mutation.
机译：温度敏感的Simian病毒（SV 40） - 转化的3T3细胞（TSSV3T3），其在32℃下生长但不在39℃时表达转化的表型，用于研究转移或移位实验期间生长行为的变化。在32℃达到或超出单层密度的TSSv3T3细胞的培养物中，DNA合成在换档后24至48小时内达到非常低的水平。当被允许在32℃的高密度生长到39℃的细胞时，不仅细胞生长停止，但在两到三天内，培养物将大量细胞流入培养基中。不可行的细胞，并且仅当随附的细胞数量减少到39℃的细胞数量减少到饱和密度的特征时，脱落均停止。剩余的附着细胞可行，并且在向32℃的变化后再次从单层生长后高细胞密度。在不同条件下，该行为与正常的3T3和野生型SV3T3细胞进行了比较。我们还使用已感染非诱变野生型SV40的细胞分离出新的TSSV3T3线。这进一步证明了这些线的温度敏感性是由于细胞而不是病毒突变。

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