RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.

B Jacquemont; B Roizman

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RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.

机译：用疱疹病毒感染细胞中的RNA合成。 X.病毒对称转录物的性质和由它们制备的双链RNA。

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HEp-2 cells infected with herpes simplex 1 virus contain RNA transcripts capable of forming double-stranded (DS) RNA on annealing. The properties of purified DS RNA were as follows (i) DS RNA is resistant to depolymerization by RNase A or T-1 in 2 times 0.15 M NaCl, plus 0.015 M sodium citrate (SSC) but not 0.1 times SSC or following thermal denaturation. (ii) The Tm of the viral DS RNA was 100 C in 0.1 times SSC. (iii) Undenatured DS RNA does not hybridize with viral DNA: upon denaturation, excess unlabeled RNA drove 50 to 55% of labeled DNA into DNA-RNA hybrid. The kinetics of hybridization indicate that the DS RNA consists of at least two populations of transcripts arising from 29 and 26% of viral DNA and differing 40-fold in molar concentration.

机译：用疱疹1病毒感染的HEP-2细胞含有能够在退火上形成双链（DS）RNA的RNA转录物。纯化的DSRNA的性质如下（I）DS RNA通过RNase A或T-1抵抗2次0.15M NaCl，加0.015μm柠檬酸钠（SSC），但不为0.1倍SSC或在热变性之后。（ii）病毒DSRNA的TM在0.1倍SSC中为100℃。（iii）未表明的DS RNA与病毒DNA不杂交：在变性时，过量的未标记的RNA将50至55％标记为DNA-RNA杂交物。杂交的动力学表明，DSRNA由29和26％的病人DNA引起的至少两个转录物群体组成，并且以摩尔浓度不同40倍。

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《Journal of Virology》 |1975年第4期|共7页
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B Jacquemont; B Roizman;
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收录信息美国《科学引文索引》(SCI);
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1. RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them. [J] . B Jacquemont, B Roizman Journal of Virology . 1975,第4期

机译：用疱疹病毒感染细胞中的RNA合成。 X.病毒对称转录物的性质和由它们制备的双链RNA。
2. RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them. [J] . B Jacquemont, B Roizman Journal of Virology . 1975,第4期

机译：用疱疹病毒感染细胞中的RNA合成。 X.病毒对称转录物的性质和由它们制备的双链RNA。
3. Lifetimes of mRNA molecules directing the synthesis of viral proteins in herpes simplex virus-infected cells. [J] . R L Ward, J G Stevens Journal of Virology . 1975,第1期

机译：MRNA分子的寿命引导在单纯疱疹病毒感染细胞中的病毒蛋白合成。
4. Anti-Viral Activity of Norfloxacin and Ampicillin and Dibutyltin Polymers Derived From Norfloxacin And Ampicillin Against Reovirus ST3, Vaccinia Virus, Herpes Simplex Virus (HSV-1) And Varicella Zoster Virus (VZV) [C] . Michael R. Ronera, Charles E. Carraher, Joanne L. Roehra Division of Polymeric Material: Science and Engineering American Chemical Society meeting . 2004

机译：诺氟沙星的抗病毒活性和氨苄青霉素衍生自Notfloxacin和Ampicillin对抗Reovirus ST3，痘苗病毒，单纯疱疹病毒（HSV-1）和水痘带状疱疹病毒（VZV）的抗毒素
5. Studies on the export of viral mRNA by the herpes simplex virus type 1 regulatory protein ICP27. [D] . Johnson, Lisa A. 2009

机译：单纯疱疹病毒1型调节蛋白ICP27出口病毒mRNA的研究。
6. RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them. [O] . B Jacquemont, B Roizman 1975

机译：单纯疱疹病毒感染的细胞中的RNA合成。 X.病毒对称转录本和由其制备的双链RNA的性质。
7. RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them [O] . B Jacquemont, B Roizman 1975

机译：用疱疹病毒感染细胞中的RNA合成。 X.病毒对称转录物的性质和由它们制备的双链RNA

RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.

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