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首页> 外文期刊>The biochemical journal >High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts
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High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts

机译：高速率[1-14℃]乙酸盐掺入分离的菠菜叶绿体中

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pSpinach chloroplasts, isolated by techniques yielding preparations with high O2- evolving activity, showed rates of light-dependent acetate incorporation into lipids 3-4 fold higher than any previously reported. Incorporation rates as high as 500 nmol of acetate/h per mg of chlorophyll were measured in buffered sorbitol solutions containing only NaHCO3 and [1-14C]acetate, and as high as 800 nmol/h per mg of chlorophyll when 0.13 mM-Triton X-100 was also included in the reaction media. The fatty acids synthesized were predominantly oleic (70-80% of the total fatty acid radioactivity) and palmitic (20-25%) with only minor amounts (1-5%) of linoleic acid. Linolenic acid synthesis was not detected in the system in vitro. Free fatty acids accounted for 70-90% of the radioactivity incorporated and the remainder was shared fairly evenly between 1,2-diacylglycerols and polar lipids. Oleic acid constituted 80-90% of the free fatty acids synthesized, but the diacylglycerols and polar lipids contained slightly more palmitic acid than oleic acid. Triton X-100 stimulated the synthesis of diacylglycerols 3-6 fold, but stimulated free fatty acid synthesis only 1-1.5-fold. Added glycerol 1-phosphate stimulated both the synthesis of diacylglycerols and palmitic acid relative to oleic acid, but did not increase acetate incorporation into total chloroplast lipids. CoA and ATP, when added separately, stimulated acetate incorporation into chloroplast lipids to variable extents and had no effect on the types of lipid synthesized, but when added together resulted in 34% of the incorporated acetate appearing in long-chain acyl-CoA. Pyruvate was a much less effective precursor of chloroplast fatty acids than was acetate./p
机译：菠菜叶绿体，通过技术分离的技术，得到具有高O2-进化活性的制剂，显示出光依赖性醋酸盐的速率掺入脂质中的脂质3-4倍高于先前报道的脂质。在仅含有NaHCO3和[1-14℃]乙酸盐的缓冲山梨糖醇溶液中测量高达500nmol氯藻醇的乙酸盐/ h的掺入速率，并且当0.13mm-triton x时，每mg叶绿素的高达800nmol / h -100也包含在反应介质中。合成的脂肪酸主要是油酸（70-80％的总脂肪酸放射性）和棕榈酸（20-25％），仅具有少量（1-5％）的亚油酸。在体外未检测到亚麻酸合成。自由脂肪酸占掺入的70-90％的放射性，其余部分在1,2-二酰基甘油和极性脂质之间相当均匀地共用。油酸构成了80-90％的游离脂肪酸，但二酰基甘油和极性脂质含有比油酸稍微更多的棕榈酸。 Triton X-100刺激二酰基甘油的合成3-6倍，但刺激游离脂肪酸合成仅1-1.5倍。加入甘油1-磷酸盐相对于油酸的二酰基甘油和棕榈酸的合成刺激，但未将乙酸盐掺入全叶绿体脂质中。 COA和ATP，当分开添加时，将醋酸盐掺入叶绿体脂质中至可变的范围，并且对合成的脂质的类型没有影响，但是当加入时，在长链酰基上出现的34％的乙酸乙酸乙酸酯。丙酮酸是叶绿体脂肪酸的效率低于乙酸盐。

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