• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Hybridization of mRNA from adenovirus-transformed cells to segments of the adenovirus genome.
【24h】

Hybridization of mRNA from adenovirus-transformed cells to segments of the adenovirus genome.

机译：将mRNA与腺​​病毒转化细胞的杂交杂交给腺病毒基因组的区段。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cytoplasmic viral-specific RNA from the 8617 line of adenovirus type 2 (Ad2) transformed rat cells was hybridized to DNA fragments derived either by Eco-RI or Sma-I restriction endonucleases. Three discrete classes of viral RNA (26S, 20S, and 15S) were detected by hybridization of total cytoplasmic RNA with the fragment, and all of these RNA molecules also hybridized to the much smaller Sma-E fragment of Ad2 DNA. Poly (A)-containing RNA (exposed to 90% formamide) contained only the 20S and 15S mRNA species, suggesting that the 26S RNA might be an aggregated species. Viral RNA of the same size derived from the same regions of the genome has not been detected in lytically infected cells, suggesting some change in the pattern of transcription or RNA processing of the Ad2 DNA in the integrated state.
机译：从8617系腺病毒2（Ad2）转化的大鼠细胞的细胞质病毒特异性RNA与Eco-Ri或SMA-I限制性内切核酸酶杂交至衍生的DNA片段。通过用片段的总细胞质RNA杂交来检测三类的三类病毒RNA（26s，20s和15s），并且所有这些RNA分子也与AD2 DNA的更小的SMA-E片段杂交。聚（a） - 甲状腺肿（暴露于90％甲酰胺）仅含有20s和15s mRNA物种，表明26s的RNA可能是聚集物种。在裂解的细胞中未检测到源自基因组的相同区域的相同区域的相同区域的病毒RNA，表明综合状态下AD2 DNA的转录或RNA处理模式的一些变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号