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首页> 外文期刊>Journal of Virology >Close association between shape alteration and loss of immunity to superinfection in a wild-type Klebsiella pneumoniae stable lysogen which can be both immune and nonimmune to superinfection.
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Close association between shape alteration and loss of immunity to superinfection in a wild-type Klebsiella pneumoniae stable lysogen which can be both immune and nonimmune to superinfection.

机译：在野生型Klebsiella肺炎稳定的溶酶体中，形状改变与免疫损失之间的关联，可以是免疫和非免疫性的。

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Klebsiella pneumoniae MirM7 is a wild-type strain which grows as cocci at pH 7 and above and as rods at pH 6.5 and below. Cultures of this strain and an auxotrophic derivative, MirM7b, have been found to undergo spontaneous lysis after purification from possible contaminating viruses. Lysates always contained two phages, FR2 and AP3, most often at high titers. FR2 and AP3 plated with the same efficiency on both MirM7b and K59 (another K. pneumoniae strain sensitive to FR2 and AP3) and lysogenized 45 and 54% of the K59-infected cells, respectively. These findings raise the possibility that MirM7b is lysogenic for FR2 and AP3, although nonimmune to their superinfection. The fact that mitomycin C and N-methyl-N'-nitro-N-nitrosoguanidine can induce phages FR2 and AP3 from MirM7b confirmed this possibility. When MirM7b was infected with FR2 several strains immune to FR2 and AP3, which were all rod shaped, were obtained. Furthermore, 19 derivatives, rod shaped at all pH's have been isolated from MirM7b. They were all immune to both FR2 and AP3. From mating experiments between the MirM7b donor derivative, strain M720, and either K59 or MirCV5, a rod-shaped MirM7b derivative cured from the prophages, cysteine recombinants were obtained which were most often (80%) immune to FR2 and AP3. Nonimmune and still lysogenic recombinants were obtained by mating M720 with a rod-shaped immune MirM7b derivative; the majority of the non-immune strains maintained the rod shape. Five coccus-shaped recombinants were also isolated; they were nonimmune to superinfection. Several physiological properties of strain MirM7b and the other nonimmune coccal recombinants have been studied in comparison with those of the rod-shaped immune derivatives. All of the coccal strains have shown several alterations with respect to the rods. The role of possible derepressed prophage genes in the various physiological alterations of MirM7 is discussed, and the analogies between this system and those of vertebrate cells transformed by proviruses are stressed.
机译：Klebsiella Pneumoniae Mirm7是一种野生型菌株，其在pH 7及以上的COCC1中生长，并且在pH 6.5和以下杆。已经发现该菌株和营养营养衍生物的培养物和营养营养衍生物MIRM7B在从可能的污染病毒纯化后经过自发性裂解。裂解物总是含有两种噬菌体，FR2和AP3，最常是高滴度。 FR2和AP3在MIRM7B和K59（对FR2和AP3敏感的另一K.肺炎肺炎）和溶酶化45和54％的K59感染细胞上的效率相同。这些发现引发了Mirm7b对于FR2和AP3的溶血性的可能性，尽管它们的超育仍然是非免疫性的。丝裂霉素C和N-甲基-N'-NITRO-N-亚硝基胍可以诱导MIRM7B的噬菌体FR2和AP3的事实证实了这种可能性。当MIRM7B感染FR2的几种菌株时，得到的菌株和AP3，它们是所有杆状的AP3。此外，19种衍生物，在所有pH下都是从MIRM7B中分离的。它们都对FR2和AP3都免疫。从MIRM7B供体衍生物，菌株M720和K59或MIRCV5之间的交配实验，获得从前位固化的棒状MIRM7B衍生物，获得半胱氨酸重组剂，其最常（80％）免受FR2和AP3。通过配合M720与棒状免疫细胞7B衍生物配合NOMMUNE和仍有溶血性重组。大多数非免疫菌株保持杆状。还分离出五种Coccus形重组剂;它们是超级融合的非imune。研究了菌株Mirm7b的几种生理特性和其他非免疫重组体的杆状免疫衍生物的重组。所有的Coccal菌株都示出了关于杆的几个改变。讨论了可能的重症的前血管基因在Mirm7各种生理改变中的作用，并强调该系统与潜水术转化的脊椎动物细胞之间的类比。

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