In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions.

M S Collett; A J Faras

首页> 外文期刊>Journal of Virology >In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions.

【24h】

In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions.

机译：通过RNA定向的DNA聚合酶在体外转录Theavian Oncornavirus基因组：在重建的酶促反应中合成的DNA转录物分析。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We have analyzed the DNA products synthesized in vitro in reconstructed reactions containing purified avian oncornavirus genome RNA and RNA-directed DNA polymerase. The results of these studies indicate that: (i) the initial DNA product synthesized on either 70S RNA or reconstituted 35S RNA-tRNAtrp template - primer complexes in the presence of low concentrations of deoxynucleoside triphosphates consists of several discrete size classes, none of which exceed 200 nucleotides in length; (ii) large DNA transcripts (about 2,000 nucleotides) can be synthesized on both 70S RNA and the 35S RNA - tRNAtrp complex by increasing the deoxynucleoside triphosphate concentration; and (iii) DNA synthesized by detergent-disrupted virus is considerably longer than DNA synthesized in reconstructed reactions.

机译：我们已经分析了在含有纯化的禽血管基因组RNA和RNA定向的DNA聚合酶的重建反应中体外合成的DNA产品。这些研究的结果表明：（i）在70秒RNA或重构的35s RNA-TRNATRP模板 - 引物在低浓度的脱氧核苷三磷酸盐中合成的初始DNA产物由几个离散尺寸的等级组成，其中没有长度为200个核苷酸; （ii）通过增加脱氧核苷三磷酸浓度，可以在70s RNA和35s RNA-Trnatrp复合物上合成大DNA转录物（约2,000个核苷酸）; （III）通过洗涤剂破坏的病毒合成的DNA显着比重建反应中合成的DNA长。

著录项

来源
《Journal of Virology》 |1977年第1期|共11页
作者
M S Collett; A J Faras;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);
原文格式 PDF
正文语种
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions. [J] . M S Collett, A J Faras Journal of Virology . 1977,第1期

机译：通过RNA定向的DNA聚合酶在体外转录Theavian Oncornavirus基因组：在重建的酶促反应中合成的DNA转录物分析。
2. In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions. [J] . M S Collett, A J Faras Journal of Virology . 1977,第1期

机译：通过RNA定向的DNA聚合酶在体外转录Theavian Oncornavirus基因组：在重建的酶促反应中合成的DNA转录物分析。
3. In vitro transcription of the avian oncornavirus genome by the RNA-directed DNA polymerase: effect of actinomycin D on the extent of transcription. [J] . C H Clayman, M S Collett, A J Faras Journal of Virology . 1977,第1期

机译：通过RNA定向的DNA聚合酶对禽类血管基因组的体外转录：放线霉素D对转录程度的影响。
4. Genome-Wide Analysis of Transcription Factor Binding Sites and Their Characteristic DNA Structures [C] . Zhiming Dai, Dongliang Guo, Xianhua Dai, International symposium on bioinformatics research and applications . 2014

机译：转录因子结合位点及其特征DNA结构的全基因组分析
5. The Origin and Evolution of Plant-Specific RNA Polymerases and Genes Involved in RNA-Directed DNA Methylation [D] . Trujillo, Joshua Tyler. 2019

机译：植物特异性RNA聚合酶的起源和演变和参与RNA定向的DNA甲基化的基因
6. In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions. [O] . M S Collett, A J Faras 1977

机译：RNA定向的DNA聚合酶体外转录theavian癌病毒基因组：分析在重建的酶促反应中合成的DNA转录本。
7. In vitro transcription of the avian oncornavirus genome by the RNA-directed DNA polymerase: effect of actinomycin D on the extent of transcription [O] . C H Clayman, M S Collett, A J Faras 1977

机译：通过RNA定向的DNA聚合酶在禽类oncornavirus基因组的体外转录：放线菌素D对转录程度的影响

In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions.

摘要

著录项

相似文献

相关主题

期刊订阅