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首页> 外文期刊>Journal of Virology >Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup.
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Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup.

机译：猿猴病毒40-聚瘤亚组乳头病毒的常见结构抗原。

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摘要

An antigenic determinant common to the major capsid polypeptide (VP1) of simian virus 40 (SV40) and polyoma virus is described. Antisera prepared against intact viral particles reacted only with cells infected with the homologous virus by immunofluorescence tests (IF). However, antisera prepared against disrupted SV40 particles reacted in IF with both polyoma- and SV40-infected permissive cells. The cross-reaction with polyoma was localized to VP1 by the following evidence. (i) The IF cross-reaction was inhibited by preincubation of the antiserum with purified SV40 VP1; (ii) purified radiolabeled polyoma VP1 was precipitated by the cross-reactive serum, and this reaction was inhibited by unlabeled SV40 VP1; (iii) other antisera prepared against purified SV40 VP1 or polyoma VP1 reacted in IF with both SV40- and polyma-infected permissive cells. These cross-reacting antisera also reacted in IF with permissive cells infected with BK virus, rabbit kidney vacuolating virus, and the stumptailed macaque virus, suggesting that all members of the polyoma-SV40 subgroup share a common antigenic determinant located in their major capsid polypeptides.
机译：描述了Simian病毒40（SV40）和聚环病毒的主要衣壳多肽（VP1）共同的抗原决定簇。反对完整病毒颗粒制备的抗血清仅通过免疫荧光试验（IF）与感染同源病毒感染的细胞反应。然而，抗血清反对破坏的SV40颗粒，如果多瘤和SV40感染的允许细胞反应。通过以下证据将与多瘤的交叉反应定位于VP1。 （i）如果通过用纯化的SV40 VP1预孵育抗血清抑制了IF交叉反应; （ii）通过交叉反应性血清沉淀出纯化的放射性标记的聚环VP1，通过未标记的SV40 VP1抑制该反应; （iii）与SV40-和Polyma感染的允许细胞一起反应的纯化SV40 VP1或PolyoMa VP1的其他抗血清。这些交叉反应抗血清还反应，如果允许用BK病毒，兔肾脏真空病毒和棘手的短尾猿病毒感染允许细胞，则表明聚瘤-SV40亚组​​的所有成员共用位于其主要衣壳多肽中的常见抗原决定蛋白。

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