• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNA's.
【24h】

RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNA's.

机译：RNase III切割囊泡口炎病毒基因组长RNA，但未能切割病毒mRNA。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The procaryotic RNA processing enzyme RNase III (endoribonuclease III [EC 3.1.4.24]) was used to probe vesicular stomatitis virus (VSV) RNAs for specific sites that could be recognized and cleaved. The effect of the enzyme on the RNAs was monitored by measuring their subsequent migration in denaturing agarose-urea gels. VSV virion RNA (negative strand; Mr, 4 X 10(6)) was cleaved by the enzyme to yield a set of discrete fragments which ranged on size from 3.5 X 10(6) to 0.2 X 10(6) daltons. The cleavage was a function of enzyme concentration, salt concentration, and time. A maximum of 20 to 22 fragments was generated under conditions of low enzyme concentration or short times of incubation. VSV genome-length intracellular RNA of both + and - polarity was also cleaved by RNase III. In contrast to the findings with virion-length RNA, however, the migration rates of VSV mRNA's purified by chromatography on polyuridylic acid-Sepharose were unaffected by treatment with RNase III. These results show that specific sites in the virion RNA and its full-length complement can be recognized by RNase III. Sites of this type are not present in the polyadenylic acid-containing mRNA, however.
机译：原律RNA加工酶RNase III（覆代核酸酶III [ec 3.1.4.24]）用于探测可以识别和切割的特异性位点的囊泡口炎病毒（VSV）RNA。通过测量变性琼脂糖 - 尿素凝胶的随后迁移，监测酶对RNA上的影响。 VSV病毒虫RNA（负股; MR，4×10（6））被酶切割，得到一组离散片段，其范围为3.5×10（6）至0.2×10（6）道尔顿。切割是酶浓度，盐浓度和时间的函数。在低酶浓度或孵育短次的条件下产生最多20至22片碎片。 +和 - 极性的VSV基因组长度的细胞内RNA也被RNase III裂解。然而，与具有病毒素长度RNA的发现相比，通过用RNase III的处理，通过色谱法纯化的VSV mRNA的迁移率未受rNase III处理的影响。这些结果表明，RNase III可以识别维斯蒂氏菌NA和其全长补体中的特定位点。然而，这种类型的位点不存在于含多腺苷酸的mRNA中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号