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首页> 外文期刊>Journal of Virology >Elongation of DNA complementary to the 5' end of the avian sarcoma virus genome by the virion-associated RNA-dependent DNA polymerase.
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Elongation of DNA complementary to the 5' end of the avian sarcoma virus genome by the virion-associated RNA-dependent DNA polymerase.

机译：DNA的伸长率互补禽类相关的RNA依赖性DNA聚合酶的禽类病毒基因组的5'末端。

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摘要

RNA-dependent DNA synthesis in a virion-associated reaction has been described as being dependent upon the detergent concentration used for disruption of the virion. In this study, the Triton X-100 concentration was found to affect the elongation of the initially synthesized DNA complementary to the last approximately 100 nucleotides at the 5' end of the RNA (cDNA100). Whereas elongation of cDNA100 increased with time of incubation at the optimal detergent concentration, this process was retarded at higher detergent concentrations. At the optimal detergent concentration, elongated DNA was of low chemical complexity, indicating that extension of cDNA100 occurred at a unique site on the RNA. Higher than optimal detergent concentrations resulted in nonspecific elongation and in DNA of high chemical complexity. This was shown by oligopyrimidine tract analysis. Furthermore, actinomycin D was observed to inhibit the elongation of cDNA100 at the optimal detergent concentration. The nature of the elongation process was elucidated by analysis of DNA synthesized in a virion-associated reaction in the presence of bacteriophage Qbeta RNA. At the optimal detergent concentration DNA complementary only to avian sarcoma virus RNA was synthesized, whereas at higher concentrations DNA was copied from both avian sarcoma virus and Qbeta RNA. We conclude that the elongation mechanism of cDNA100 is affected by the detergent concentration and elongation is unspecific at higher than optimal detergent concentrations. The mechanism by which the nonionic detergent stimulates DNA synthesis has not yet been resolve. We assume that other factors in addition to DNA polymerase are involved in elongation of cDNA100.
机译：已经描述了在病毒中相关反应中的RNA依赖性DNA合成依赖于用于破坏病毒粒的洗涤剂浓度。在该研究中，发现TRITON X-100浓度影响最初合成的DNA互补互补于RNA的5'末端的最后约100个核苷酸（cDNA100）。虽然CDNA100的伸长随着在最佳洗涤剂浓度的孵育时增加，但是在较高的洗涤剂浓度下延迟该方法。在最佳洗涤剂浓度下，细长的DNA具有低化学复杂性，表明CDNA100的延伸发生在RNA上的独特位点。高于最佳洗涤剂浓度导致非特异性伸长和高化学复杂性的DNA。这是由寡替米啶丁分析显示的。此外，观察到放射霉素D以抑制在最佳洗涤剂浓度下CDNA100的伸长。通过在噬菌体Qbeta RNA存在下分析在病毒藻相关反应中合成的DNA来阐明延伸过程的性质。在最佳洗涤剂浓度下，合成仅用于禽Sarcoma病毒RNA的DNA互补，而在较高浓度下，DNA从禽Sarcoma病毒和Qbeta RNA复制。我们得出结论，CDNA100的伸长机制受洗涤剂浓度的影响，并且伸长率是不特异性的高于最佳的洗涤剂浓度。非离子洗涤剂刺激DNA合成的机制尚未解决。我们假设除DNA聚合酶之外的其他因素也参与CDNA100的伸长率。

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