• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Genetic Analysis of a Baculovirus, Autographa californica Nuclear Polyhedrosis Virus I. Isolation of Temperature-Sensitive Mutants and Assortment into Complementation Groups
【24h】

Genetic Analysis of a Baculovirus, Autographa californica Nuclear Polyhedrosis Virus I. Isolation of Temperature-Sensitive Mutants and Assortment into Complementation Groups

机译：杆状病毒的遗传分析，术，术，核核多角形病毒I.将温度敏感突变体和分类分离成互补基团

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Temperature-sensitive (ts) mutants were isolated from the baculovirus Autographa californica (alfalfa looper) MNPV, grown in Spodoptera frugiperda (fall armyworm) cells in the presence of N-methyl-N′-nitro-N-nitrosoguanidine. Of 567 plaque isolates screened, 27 were temperature sensitive (ts), representing a mutation frequency of 4.8%. Ten ts mutants were studied in detail: six failed to yield nonoccluded virus at 33°C (NOV mutants), whereas the other four produced nonoccluded virus but were restricted in formation of polyhedra at 33°C (Poly mutants). One of the six NOV mutants failed to synthesize viral DNA. Reversion and leak frequencies were determined, and the mutants were assorted into complementation groups based on the yield of polyhedrin synthesis in cells coinfected with pairs of mutants at 33°C, as measured by radioimmunoassay. For NOV mutants, complementation indexes were also based on virus yield and were consistent with those based on polyhedrin synthesis. Nine mutants were assorted into five complementation groups. One mutant remained unclassified.
机译：从Baculovirus Autographa Californica （Alfalfa换页） m npv中分离出温度敏感（ ts ）突变体，在 spodoptera frugiperda中生长< / EM>（秋季肺部）细胞在 N -methyl- N' -NITRO- N -NITROSOGUANIDINE。在567个斑点分离株筛选，27例为温度敏感（ TS ），表示突变频率为4.8％。详细研究了十个 TS 突变体：六未能在33℃（ Nov 突变体）下产生非核状病毒，而另外四种产生的非核状病毒，但受限制Polyhedra在33℃（ Poly 突变体）。六个 Nov 突变体中的一种未能合成病毒DNA。确定逆转和泄漏频率，并且通过放射免疫测定法测量，基于在33℃的突变体的细胞中屈服于聚溴苯胺合成的产量，将突变体分为互补基团。对于 Nov 突变体，互补指标也基于病毒产量，并且与基于多肾上腺素合成的人一致。九个突变体被分为五种互补组。一个突变体保持未分类。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号