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首页> 外文期刊>Journal of Virology >Isolation, Complementation, and Initial Characterization of Temperature-Sensitive Mutants of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus
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Isolation, Complementation, and Initial Characterization of Temperature-Sensitive Mutants of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus

机译：杆状病毒综述Californica核多面体病毒温度敏感突变体的分离，互补和初始表征

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摘要

Sixteen temperature-sensitive mutants of Autographa californica nuclear polyhedrosis virus were isolated. Several interesting phenotypes were observed. A large proportion of the mutants were unable to form polyhedral occlusion bodies (polyhedra) at the nonpermissive temperature (32.5°C). At 32.5°C, one mutant formed plaques in which the cells lacked polyhedra. Another mutant type was defective in the production of progeny extracellular nonoccluded virus and produced a “plaque” consisting of only a single cell containing polyhedra at 32.5°C. One mutant was defective in plaque formation, progeny nonoccluded virus formation, and polyhedra formation at 32.5°C. Several mutants produced nonoccluded virus but failed to produce plaques or polyhedra at 32.5°C. Other phenotypes were also distinguished. Complementation analyses, performed by either measuring the increase in extracellular nonoccluded virus formation or by observing polyhedra formation in mixed infections at 32.5°C, indicated the presence of 15 complementation groups. A high frequency of recombination was observed. Four of the mutants were found to be host dependent in their temperature sensitivity for polyhedra formation.
机译：分离出90个温度敏感突变体 upotographa californica 核多面体病毒。观察到几种有趣的表型。大部分突变体在非智能温度（32.5℃）下不能形成多面体闭塞体（多面体）。在32.5℃，一个突变体形成的斑块，其中细胞缺乏多面体。在后代细胞外非核状病毒的生产中，另一种突变体类型有缺陷，并制备了由32.5℃的单个细胞的单个细胞组成的“斑块”。一种突变体在斑块形成，后代非核状病毒形成和32.5℃的斑块形成。几个突变体产生的不粘合的病毒，但未能在32.5℃下产生斑块或多面体。其他表型也有所区分。通过测量细胞外非核状病毒形成的增加或通过观察32.5℃的混合感染中的多面体形成来进行的互补分析。表明存在15个互补基团。观察到高频率的重组。发现四个突变体是宿主依赖于它们对多面体形成的温度敏感性。

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