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首页> 外文期刊>The biochemical journal >Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy
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Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy

机译：尿苷与尿苷与核心肝脏核糖核酸标记与核糖核酸标记的合成代谢及其与核糖核酸标记

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pThe balance between anabolism and catabolism of [5-sup3/supH]uridine was studied in the mouse after partial hepatectomy. Labelling of RNA and UDP-glucose was determined and evaluated in relation to changes in the specific radioactivity of UTP. The amounts of labelled catabolic products of uridine were increased several-fold in liver and blood after partial hepatectomy. The specific radioactivity of RNA decreased to about 60% of the control value at 6h and was in the same range as that of control liver at 24h after operation. Decreased labelling of RNA and UDP-glucose was attributable to decreased specific radioactivity of UTP. No changes in the size of the UTP pool or in the balance between uridine anabolism and catabolism were found that could explain the decreased specific radioactivity of UTP. Rather, the alterations in the labelling of this metabolite induced by the partial hepatectomy may be related to decreased phosphorylating capacity in the liver cells and/or dilution of the labelled precursor in an expanded uridine pool. The enhanced amounts of uridine catabolic products in liver and blood were probably a consequence of accumulation and altered incorporation of the metabolites from the blood into the liver cells. Despite the increased amounts of labelled catabolic products and the decreased labelling of RNA, the results reported here actually suggest decreased uridine catabolism and slightly increased RNA synthesis in mouse liver after partial hepatectomy. The results stress the importance of proper controls in determination of nucleic acid synthesis and in metabolic studies by use of labelled precursors./p
机译：在部分肝切除术后，在小鼠中研究了[5- 3 h]尿苷的原料和分解代谢之间的平衡。测定RNA和UDP-葡萄糖的标记，并与UTP的特定放射性的变化有关。在部分肝切除术后肝脏和血液中标记的尿苷的标记分解代谢产物的量增加了几折。 RNA的特定放射性在6小时下降低至约60％的对照值，并且在操作后24小时与对照肝脏的范围相同。降低RNA和UDP-葡萄糖的标记可归因于UTP的特异性放射性降低。没有发现UTP池大小的变化或在尿苷的尿苷与分解代谢之间的平衡中，可以解释UTP的细细放射性降低。相反，部分肝切除术诱导的这种代谢物标记的改变可能与肝细胞中的磷酸化能力降低，和/或在膨胀的尿苷池中稀释标记的前体。肝脏和血液中尿苷分解代谢产物的增强量可能是积累的结果，并改变了代谢物从血液中的代谢物掺入肝细胞中。尽管标记的分解代谢产物量增加和RNA的标记下降，但在此报告的结果实际上表明尿苷分解代谢和部分肝切除术后小鼠肝脏的RNA合成略微增加。结果应力通过使用标记的前体来强调适当对照在测定核酸合成和代谢研究中的重要性。

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