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首页> 外文期刊>Journal of Virology >DNA binding properties of simian virus 40 T-antigens synthesized in vivo and in vitro.
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DNA binding properties of simian virus 40 T-antigens synthesized in vivo and in vitro.

机译：体内和体外合成的Simian病毒40 T-抗原的DNA结合特性。

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摘要

Simian virus 40 large T- and small t-antigens have been shown previously to share immunological determinants and common sequences and to have roles in virus-induced cell transformation. However, only large T-antigen is a DNA binding protein. Under all conditions tested, small t-antigen did not interact with DNA. Large T-antigen synthesized in infected cells bound to both native calf thymus and simian virus 40 DNAs. As its binding efficiency was less than 100%, it is likely that there are different forms of T-antigen which vary in their affinity for DNA. Large T-antigen synthesized in cell-free protein-synthesizing systems primed by simian virus 40 mRNA also bound to DNA-cellulose, whereas small t-antigen similarly synthesized in vitro did not. An 82,000-molecular-weight T-antigen polypeptide synthesized in cell-free protein-synthesizing systems primed by simian virus 40 complementary RNA transcribed in vitro from simian virus 40 DNA by Escherichia coli RNA polymerase bound efficiently to simian virus 40 DNA. As this product did not share sequences with the small t-antigen, it can be concluded that the amino-terminal portion of the T-antigen is not required for some of its specific DNA binding properties.
机译：先前已显示Simian病毒40大型T-和小T抗原以分享免疫学决定因素和常见序列并在病毒诱导的细胞转化中具有作用。然而，只有大的T-抗原是DNA结合蛋白。在所有测试的条件下，小型T-抗原与DNA没有相互作用。在与天然小牛胸腺和Simian病毒40dNA合并的感染细胞中合成的大T-抗原。随着其结合效率小于100％，可能存在不同形式的T-抗原，其对DNA的亲和力变化。在由Simian病毒40 mRNA引发的无细胞蛋白合成系统中合成的大T-抗原也与DNA-纤维素结合，而在体外相似合成的小T抗原没有。在由猿猴病毒40DNA的体外转录的Simian病毒40互补RNA以有效地与Simian病毒40dNa合并，合成的82,000分子量的T-抗原多肽。由于本产品未与小T-抗原共享序列，可以得出结论，其特定DNA结合特性的一些特异性DNA结合特性不需要T-抗原的氨基末端部分。

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