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首页> 外文期刊>Journal of Virology >Partial replication of UV-irradiated T4 bacteriophage DNA results in amplification of specific genetic areas.
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Partial replication of UV-irradiated T4 bacteriophage DNA results in amplification of specific genetic areas.

机译：紫外线辐照的T4噬菌体DNA的部分复制导致特定遗传区域的扩增。

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摘要

Upon infection of Escherichia coli with bromodeoxyuridine-labeled t4 phage that had received 10 lethal hits of UV irradiation, a sizable amount of phage DNA was synthesized (approximately 36 phage equivalent units of DNA per infected bacterium), although very little multiplicity reactivation occurs. This progeny DNA was isolated and analyzed. This DNA was biased in its genetic representation, as shown by hybridization to cloned segments of the T4 genome immobilized on nitrocellulose filters. Preferentially amplified areas corresponded to regions containing origins of T4 DNA replication. The size of the progeny DNA increased with time after infection, possibly due to recombination between partial replicas and nonreplicated subunits or due to the gradual overcoming of the UV damage. As the size of the progeny DNA increased, all of the genes were more equally represented, resulting in a decrease in the genetic bias. Amplification of specific genetic areas was also observed upon infection with UV-irradiated, nonbromodeoxyuridine-substituted (light) phage. However, the genetic bias observed in this case was not as great as that observed with bromodeoxyuridine-substituted phage. This is most likely due to the higher efficiency of multiplicity reactivation of the light phage.
机译：在用溴肟尿苷标记的T4噬菌体感染大肠杆菌上，所述T4噬菌体接受了10个致命辐射的致死次数，合成了大量的噬菌体DNA（每次感染细菌的大约36个噬菌体当量单位），但是发生了很少的多重再活化。将这种后代DNA分离并分析。该DNA在其遗传形式中偏置，如杂交所示，以固定在硝酸纤维素过滤器上的T4基因组的克隆区段所示。优选扩增的区域对应于含有T4 DNA复制的渊源的区域。后代DNA的大小随着感染后的时间而增加，可能是由于部分复制品和非重叠亚基之间的重组或由于逐渐克服UV损伤。随着后代DNA的尺寸增加，所有基因更像地表示，导致遗传偏压的降低。还在用紫外线辐照的非溴酸核酰氨酸取代（Light）噬菌体感染后观察到特异性遗传区域的扩增。然而，在这种情况下观察到的遗传偏差并不与用溴肟尿苷取代的噬菌体观察到的遗传偏差。这很可能是由于光噬菌体的多重再现效率较高。

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