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首页> 外文期刊>Journal of Virology >Monoclonal antibodies to the transformation-specific glycoprotein encoded by the feline retroviral oncogene v-fms.
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Monoclonal antibodies to the transformation-specific glycoprotein encoded by the feline retroviral oncogene v-fms.

机译：单克隆抗体对由猫逆转录病毒癌基因V-FMS编码的转化特异性糖蛋白。

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Monoclonal antibodies prepared to epitopes encoded by the transforming gene (v-fms) of the McDonough strain of feline sarcoma virus were used to study v-fms-coded antigens in feline sarcoma virus-transformed rat and mink cells. These antibodies reacted with three different polypeptides (gP180gag-fms, gp140fms, and gp120fms), all of which were shown to be glycosylated. Protein blotting with [125I]-labeled monoclonal immunoglobulin G's was used to determine the relative steady-state levels of these glycoproteins in transformed cells and showed that gp120 and gp140 were the predominant products. Immunofluorescence assays and subcellular fractionation experiments localized these molecules to the cytoplasm of transformed cells in quantitative association with sedimentable organelles. Thus, v-fms-coded glycoproteins differ both chemically and topologically from the partially characterized products of other known oncogenes and presumably transform cells by a different mechanism.
机译：用于由猫赛肉瘤病毒的McDoNouge菌株的转化基因（V-FMS）编码的表位的单克隆抗体用于研究猫赛肉瘤病毒转化的大鼠和水貂细胞中的V-FMS编码抗原。这些抗体与三种不同的多肽（GP180GAG-FMS，GP140FMS和GP120FMS）反应，所有这些抗体显示为糖基化。用[125I] - 标记的单克隆免疫球蛋白G的蛋白质印迹用于确定转化细胞中这些糖蛋白的相对稳态水平，并显示GP120和GP140是主要产物。免疫荧光测定和亚细胞分级实验将这些分子定位在与沉积的细胞器的定量结合中转化细胞的细胞质中。因此，V-FMS编码的糖蛋白在化学上和拓扑上与其他已知癌基因的部分特征的产物不同，并且可以通过不同的机制来改变细胞。

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