• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions.
【24h】

Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions.

机译：V-MOS变换活动的长终端重复增强：基本区域的识别。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The transforming efficiency of recombinant DNA clones containing the Moloney sarcoma virus v-mos sequence was enhanced by introducing the Moloney sarcoma virus long terminal repeat (LTR) in either the 5' or 3' position relative to v-mos. We analyzed the polyadenylated RNA expressed in cells transformed by these recombinant DNA clones and examined the structural integrity of integrated copies of the DNA. In each case, we demonstrated the presence of v-mos containing RNA transcripts in the polyadenylated RNA and showed that these RNA transcripts are consistent with the structure of the transfected DNA. The analysis of DNA from these transformed cells showed that the relative positions of the v-mos and LTR sequences within the transfected DNA were conserved in the integrated DNA copies. These results demonstrate that a single LTR can successfully enhance the transforming activity of v-mos from either a 5' or a 3' relative position. The results from the transfection analysis of recombinant clones containing only portions of the LTR introduced 3' to v-mos demonstrate that the essential region of the LTR responsible for the enhancement of transformation is a region within the unique 3' sequences of the LTR containing the 73-base-pair tandem repeat sequence.
机译：通过在相对于V-MOS中引入5'或3'位置的莫尼肉瘤病毒长端子重复（LTR）来增强含莫尼肉瘤病毒V-MOS序列的重组DNA克隆的转化效率。我们分析了通过这些重组DNA克隆转化的细胞中表达的聚腺苷酸化RNA，并检查了DNA的整合拷贝的结构完整性。在每种情况下，我们证明存在在多腺苷酸化的RNA中的V-MOS含有RNA转录物，并显示这些RNA转录物与转染的DNA的结构一致。来自这些转化的细胞的DNA分析表明，在集成的DNA拷贝中保守转染DNA内的V-MO和LTR序列的相对位置。这些结果表明，单个LTR可以成功增强V-MOS从5'或3'相对位置的变换活性。仅含有LTR的部分的重组克隆转染分析的结果引入3'至V-MOS的结果表明，负责转化增强的LTR的基本区域是包含该的LTR的独特3'序列内的区域。 73-碱基串联重复序列。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号