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首页> 外文期刊>Journal of Virology >Cyanogen bromide digestion of the avian myeloblastosis virus pp19 protein: isolation of an amino-terminal peptide that binds to viral RNA.
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Cyanogen bromide digestion of the avian myeloblastosis virus pp19 protein: isolation of an amino-terminal peptide that binds to viral RNA.

机译：乌亚因溴化物消化禽髓母细胞病毒PP19蛋白：与病毒RNA结合的氨基末端肽的分离。

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摘要

The avian myeloblastosis virus pp19 protein was separated from the other virus proteins by a rapid and simple purification procedure which yields milligram amounts of homogeneous protein. This protein was then fragmented by digestion with cyanogen bromide. When the mixture of the cyanogen bromide peptides was passed through a 60S avian myeloblastosis virus RNA-cellulose column, only one peptide bound with high affinity to the resin. The peptide migrated on a sodium dodecyl sulfate-polyacrylamide gel with an approximate molecular weight of 2,900 and will be referred to as the p3B peptide. This peptide was also isolated directly by chromatography of the cyanogen bromide-digested pp19 protein on a reverse-phase high-pressure liquid chromatography column. It was again the only cyanogen bromide peptide of the pp19 protein that bound to the RNA affinity resin. The p3B peptide is a basic peptide, as was seen by its rapid migration on acid-urea-polyacrylamide gels and its amino acid composition. A partial amino acid sequence analysis of the p3B peptide indicated that it was derived from the amino terminus of the intact protein. Although the p3B peptide bound to 60S RNA, it did not demonstrate the selective binding of native pp19 to regions of the RNA containing secondary structure.
机译：通过快速和简单的纯化方法与其他病毒蛋白分离禽髓霉菌病毒PP19蛋白，其产生毫克均相蛋白质。然后通过用氰基溴化物消化来破碎该蛋白质。当氰基染色肽的混合物通过60s禽血吸虫母细胞核柱时，只有一种与树脂具有高亲和力的肽。肽在十二烷基硫酸钠 - 聚丙烯酰胺凝胶上迁移，近似分子量为2,900，并将被称为P3b肽。该肽也通过在反相高压液相色谱柱上通过氰基溴化物消化的PP19蛋白的色谱分离出直接分离。再次是与RNA亲和树脂结合的PP19蛋白的唯一氰基溴化物肽。 P3B肽是碱性肽，如其对酸性尿素 - 聚丙烯酰胺凝胶及其氨基酸组合物的快速迁移所见。 P3B肽的部分氨基酸序列分析表明它衍生自完整蛋白质的氨基末端。尽管与60s RNA结合的P3B肽，但它没有证明天然PP19与含有二级结构的RNA区域的选择性结合。

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