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首页> 外文期刊>Journal of Virology >Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses.
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Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses.

机译：分子克隆和序列测定三种微微碱的基因组区域和基因组连接蛋白的基因组区域。

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To investigate the degree of similarity between picornavirus proteases, we cloned the genomic cDNAs of an enterovirus, echovirus 9 (strain Barty), and two rhinoviruses, serotypes 1A and 14LP, and determined the nucleotide sequence of the region which, by analogy to poliovirus, encodes the protease. The nucleotide sequence of the region encoding the genome-linked protein VPg, immediately adjacent to the protease, was also determined. Comparison of nucleotide and deduced amino acid sequences with other available picornavirus sequences showed remarkable homology in proteases and among VPgs. Three highly conserved peptide regions were identified in the protease; one of these is specific for human picornaviruses and has no obvious counterpart in encephalomyocarditis virus, foot-and-mouth disease virus, or cowpea mosaic virus proteases. Within the other two peptide regions two conserved amino acids, Cys 147 and His 161, could be the reactive residues of the active site. We used a statistical method to predict certain features of the secondary structures, such as alpha helices, beta sheets, and turns, and found many of these conformations to be conserved. The hydropathy profiles of the compared proteases were also strikingly similar. Thus, the proteases of human picornaviruses very probably have a similar three-dimensional structure.
机译：为了研究皮诺病毒蛋白酶之间的相似度，我们克隆了肠病毒，Echovirus 9（菌株Party）和两种鼻窦病毒，血清型1a和14Lp的基因组cdnas，并确定了该区域的核苷酸序列，通过比较脊髓灰质病毒，编码蛋白酶。还确定了编码邻近蛋白酶的基因组连接蛋白VPG的区域的核苷酸序列。核苷酸和推导的氨基酸序列与其他可用的微生物序列的比较在蛋白酶和VPG中显示出显着的同源性。在蛋白酶中鉴定出三个高度保守的肽区域;其中一个是人类小心病毒的特异性，并且在脑脊髓病毒，口蹄疫病毒或豇豆芥末病毒蛋白酶中没有明显的对应物。在另外两个肽区内，两个保守的氨基酸，Cys 147和他的161，可以是活性位点的反应性残余物。我们使用统计方法来预测二次结构的某些特征，例如α螺旋，β板和转弯，并且发现许多这些构象是节省的。相比之下的蛋白酶的水小疗法也令人惊醒地相似。因此，人类小心病毒的蛋白酶非常可能具有类似的三维结构。

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