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首页> 外文期刊>Journal of Virology >Bovine papillomavirus transcriptional regulation: localization of the E2-responsive elements of the long control region.
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Bovine papillomavirus transcriptional regulation: localization of the E2-responsive elements of the long control region.

机译：牛乳头瘤病毒转录调节：长控制区域的E2响应元件的定位。

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摘要

The long control region (LCR) of the bovine papillomavirus type 1 genome can function as a conditional transcriptional enhancer which can be specifically trans-activated by the viral E2 gene product. To precisely map the target(s) of this trans-activation, BAL 31 exonuclease was used to generate two overlapping series of deleted DNA segments through the LCR. These fragments were assayed for their ability to activate transcription from the enhancer-deleted simian virus 40 early promoter of pA10CAT in the presence or absence of the viral E2 gene product. Two different E2-responsive elements were localized within the LCR. The major target for E2 trans-activation (E2-responsive element 1) was mapped to a 196-base-pair fragment between nucleotides 7611 and 7806, just upstream from promoters P7940 and P89. Further deletions which destroyed or impaired enhancer function revealed that the ACCN6GGT sequence motifs at each end of E2-responsive element 1 are critical components of this element. Primer extension analysis of RNA extracted from acute transfections with plasmids containing the bovine papillomavirus type 1 LCR driving the CAT gene revealed that each of the P7940 and P89 promoters is responsive to E2 trans-activation.
机译：牛乳头瘤病毒1型基因组的长控制区域（LCR）可以用作有条件转录增强剂，其可以由病毒E2基因产物特异性激活。为了精确地映射该反式激活的靶标，使用BAL 31外切核酸酶通过LCR产生两个重叠系列缺失的DNA段。测定这些片段的能力，以便在病毒E2基因产物的存在或不存在中激活PA10CAT的早期启动子的转录的转录。两个不同的E2响应元件在LCR内定位。将E2逆激活（E2-响应元件1）的主要靶标映射到核苷酸7611和7806之间的196-碱基片段，仅在启动子P7940和P89的上游。被破坏或受损的增强功能函数的进一步缺失揭示了E2响应元件1的每个端部的ACCN6GGT序列图案是该元素的关键部件。从含有牛乳头瘤病毒1型LCR驱动的急性转染的RNA提取的RNA引物延伸分析探的猫基因显示，P7940和P89启动子中的每一个对E2反式激活反应。

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