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首页> 外文期刊>Journal of Virology >Identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with gE and binds immunoglobulin.
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Identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with gE and binds immunoglobulin.

机译：鉴定新型疱疹病毒1型诱导的糖蛋白，其与Ge复合并结合免疫球蛋白。

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We detected a glycoprotein on the surface of cells infected with herpes simplex virus type 1 (HSV-1) which, in conjunction with gE, binds immunoglobulin G (IgG). The novel glycoprotein, which has an apparent molecular mass of 70 kilodaltons and was provisionally named g70, was first detected in extracts of HSV-1-infected cells labeled by lactoperoxidase-catalyzed iodination and precipitated with rabbit sera or IgG and protein A-Sepharose. In subsequent experiments, g70 and gE were coprecipitated from extracts of HSV-1-infected cells labeled with [35S]methionine, [35S]cysteine, or 14C-amino acids. We were unable to precipitate a polypeptide analogous to g70 or gE from extracts of HSV-2-infected cells with rabbit IgG and protein A-Sepharose. Partial proteolytic peptide analysis indicated that g70 is structurally distinct from gE and gI). In addition, g70 was electrophoretically distinct from the HSV-1 Us4 glycoprotein gG. HSV-1 gE, expressed in mouse cells transfected with the gE gene, was not precipitated with rabbit IgG, nor could these cells bind radiolabeled IgG, suggesting that gE alone cannot act as an IgG (Fc) receptor. This result, coupled with the findings that gE and g70 are coprecipitated with IgG and with an anti-gE monoclonal antibody, suggests that gE and g70 form a complex which binds IgG. The electrophoretic mobilities of g70 molecules induced by different strains of HSV-1 differed markedly, arguing that g70 is encoded by the virus and is not a cellular protein induced by virus infection.
机译：我们在用疱疹病毒1（HSV-1）中检测到感染的细胞表面上的糖蛋白，其与Ge结合，结合免疫球蛋白G（IgG）。新的糖蛋白，其具有70千伏尔顿的表观分子量，并被临时名为G70，首先在用乳酰氧化酶催化碘化碘化的HSV-1感染细胞的提取物中检测，并用兔血清或IgG和蛋白A-Sepharose沉淀。在随后的实验中，将G70和GE与用[35s]甲硫氨酸，[35s]半胱氨酸或14℃-氨基酸标记的HSV-1感染细胞的提取物共沉淀。我们不能用兔IgG和蛋白A-Sepharose从HSV-2感染细胞的提取物中类似于G70或Ge类似于G70或Ge的多肽。部分蛋白水解肽分析表明G70在结构上与Ge和Gi不同）。此外，G70与HSV-1 US4糖蛋白GG电泳不同。 HSV-1Ge，在用GE基因转染的小鼠细胞中表达，用兔IgG沉淀，这些细胞也不能结合放射性标记的IgG，表明GE单独不能充当IgG（Fc）受体。该结果与GE和G70与IgG和抗GE单克隆抗体共沉淀的结果结合，表明Ge和G70形成结合IgG的复合物。由不同菌株HSV-1诱导的G70分子的电泳迁移势显着不同，争论G70由病毒编码，不是病毒感染诱导的细胞蛋白。

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