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Opines in naturally infected grapevine crown gall tumors

机译：自然感染的葡萄冠胆瘤中的奥平

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Crown gall tumors collected from naturally infected plants of 7 grapevine varieties were analysed for the presence of opines. Eighty-five of the tested 90 samples contained known Agrobacterium vitis-induced opines. Octopine was the most common, it was found in 50 samples. Twenty-eight crown galls contained nopaline and 8 were vitopine positive. There was only one tumor that contained two opines, nopaline and vitopine. Five samples were negative for the opines tested. The presence of A. vitis was confirmed in most of these tumors by PCR analysis. The opine assay may provide a simple diagnostic protocol to distinguish healthy callus tissues from crown galls as well as for the indirect identification of Agrobacterium infection.
机译：分析了从7个葡萄品种的自然感染植物中收集到的冠gall瘤中是否存在阿片类药物。测试的90个样品中有85个包含已知的农杆菌介导的阿片类药物。章鱼碱是最常见的，在50个样品中被发现。 28个冠gall中含有胭脂碱，其中8个是维托平阳性。只有一个肿瘤含有两种胭脂红和胭脂碱。五份样品对所测阿片类药物呈阴性。通过PCR分析在大多数这些肿瘤中证实了葡萄球菌的存在。鸦片测定可提供一种简单的诊断方案，以区分健康的愈伤组织和冠crown，并间接鉴定农杆菌感染。

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